![Western Blot: SMEK2 Antibody [NBP1-83882] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SMEK2-Antibody-Western-Blot-NBP1-83882-img0017.jpg "Western Blot: SMEK2 Antibody [NBP1-83882] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: GQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFNEDEEEEGKAVMPPLE |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SMEK2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-83882 | Applications | Species |
|---|---|---|
| Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SMEK2 |
![Immunocytochemistry/Immunofluorescence: SMEK2 Antibody [NBP1-83882] - Staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMEK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83882-img0008.jpg "Immunocytochemistry/Immunofluorescence: SMEK2 Antibody [NBP1-83882] - Staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Analysis in human fallopian tube and skeletal muscle tissues using NBP1-83882 antibody. Corresponding PPP4R38 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SMEK2-Antibody-Immunohistochemistry-Paraffin-NBP1-83882-img0010.jpg "Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Analysis in human fallopian tube and skeletal muscle tissues using NBP1-83882 antibody. Corresponding PPP4R38 RNA-seq data are presented for the same tissues.")
![Western Blot: SMEK2 Antibody [NBP1-83882] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/SMEK2-Antibody-Western-Blot-NBP1-83882-img0016.jpg "Western Blot: SMEK2 Antibody [NBP1-83882] - Analysis in human cell line HDLM-2.")
![Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SMEK2-Antibody-Immunohistochemistry-Paraffin-NBP1-83882-img0012.jpg "Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Staining of human cerebral cortex shows weak to moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/SMEK2-Antibody-Immunohistochemistry-Paraffin-NBP1-83882-img0013.jpg "Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Staining of human cerebral cortex shows weak to moderate nuclear positivity in neurons.")
![Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Staining of human testis shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SMEK2-Antibody-Immunohistochemistry-Paraffin-NBP1-83882-img0014.jpg "Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Staining of human testis shows moderate to strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SMEK2-Antibody-Immunohistochemistry-Paraffin-NBP1-83882-img0015.jpg "Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Western Blot Chk2 [p Thr68] Antibody [Unconjugated]](https://images.novusbio.com/images2/Chk2_AF1626_Western_Blot_5130.jpg)
![Immunocytochemistry Chk2 [p Thr68] Antibody [Unconjugated]](https://images.novusbio.com/images2/Chk2_AF1626_Immunocytochemistry__Immunofluorescence_18847.jpg)
![Flow Cytometry Annexin V Apoptosis Kit [FITC]](https://images.novusbio.com/images/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0001.jpg)
![Flow Cytometry Annexin V Apoptosis Kit [FITC]](https://images.novusbio.com/images/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0003.jpg)
![Flow Cytometry Annexin V Apoptosis Kit [FITC]](https://images.novusbio.com/images/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0004.jpg)
![Immunohistochemistry MMR/CD206/Mannose Receptor Antibody [Unconjugated]](https://images.novusbio.com/images2/MMR_AF2535_Immunohistochemistry_15450.jpg)
![Western Blot MMR/CD206/Mannose Receptor Antibody [Unconjugated]](https://images.novusbio.com/images2/MMR_AF2535_Western_Blot_12738.jpg)
![Immunohistochemistry MMR/CD206/Mannose Receptor Antibody [Unconjugated]](https://images.novusbio.com/images2/MMR_AF2535_Immunohistochemistry_20447.jpg)
![SDS-PAGE LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_719.jpg)
![Bioactivity LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_721.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/Histone_H2AX_AF2288_Western_Blot_5182.jpg)
![Simple Western Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/16395.jpg)
![Immunocytochemistry Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/Histone_H2AX_AF2288_Immunocytochemistry__Immunofluorescence_16920.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")