![Western Blot: Cdk7 Antibody [NB100-581] - Detection of mouse and rat CDK7 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0019.jpg "Western Blot: Cdk7 Antibody [NB100-581] - Detection of mouse and rat CDK7 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IP, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 300 and the C-terminus (residue 346) of human Cyclin-Dependent Kinase 7 using the numbering given in Swiss-Prot entry P50613 (GeneID 1022). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CDK7 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: Cdk7 Antibody [NB100-581] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 CDK7 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Cdk7-Antibody-Immunohistochemistry-NB100-581-img0016.jpg "Immunohistochemistry: Cdk7 Antibody [NB100-581] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 CDK7 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Western Blot: Cdk7 Antibody [NB100-581] - RIPA lysate (5 and 25 mcg/lane in A; 5 mg in B) from HeLa cells. Affinity purified rabbit anti-CDK7 antibody used at 0.1 mcg/ml for WB and at 4 mcg/5 mg lysate for IP.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0012.jpg "Western Blot: Cdk7 Antibody [NB100-581] - RIPA lysate (5 and 25 mcg/lane in A; 5 mg in B) from HeLa cells. Affinity purified rabbit anti-CDK7 antibody used at 0.1 mcg/ml for WB and at 4 mcg/5 mg lysate for IP.")
![Western Blot: Cdk7 Antibody [NB100-581] - Analysis using the Biotin conjugate of NB100-581. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0014.jpg "Western Blot: Cdk7 Antibody [NB100-581] - Analysis using the Biotin conjugate of NB100-581. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Cdk7 Antibody [NB100-581] - Analysis of Biotin conjugate of NB100-581. Whole cell lysate from HeLa (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, rabbit anti-CDK7 antibody used at 0.1 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0015.jpg "Western Blot: Cdk7 Antibody [NB100-581] - Analysis of Biotin conjugate of NB100-581. Whole cell lysate from HeLa (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, rabbit anti-CDK7 antibody used at 0.1 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time")
![Western Blot: Cdk7 Antibody [NB100-581] - Detection of Human CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK7 antibody NB100-581 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0018.jpg "Western Blot: Cdk7 Antibody [NB100-581] - Detection of Human CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK7 antibody NB100-581 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation: Cdk7 Antibody [NB100-581] - Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 (lot NB100-581-2) used for IP at 6 ug per reaction. CDK7 was also immunoprecipitated by a previous lot of this antibody NB100-581-1 For blotting immunoprecipitated CDK7, NB100-581 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Immunoprecipitation-NB100-581-img0020.jpg "Immunoprecipitation: Cdk7 Antibody [NB100-581] - Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 (lot NB100-581-2) used for IP at 6 ug per reaction. CDK7 was also immunoprecipitated by a previous lot of this antibody NB100-581-1 For blotting immunoprecipitated CDK7, NB100-581 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")