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SMC3 Antibody

Images

 
Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SMC3 Antibody [NBP2-20418] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SMC3 Antibody [NBP2-20418] - Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.
Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: PC-12 5% SDS PAGE gel, diluted at 1:2000.
Immunoprecipitation: SMC3 Antibody [NBP2-20418] - Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 ug of SMC3 antibody. Western blot analysis was performed using SMC3 antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SMC3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SMC3. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Xenopus (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for SMC3 Antibody

  • Bamacan
  • BAMSMC protein 3
  • Basement membrane-associated chondroitin proteoglycan
  • BMH
  • chondroitin sulfate proteoglycan 6 (bamacan)
  • Chondroitin sulfate proteoglycan 6
  • Chromosome-associated polypeptide
  • CSPG6structural maintenance of chromosomes protein 3
  • hCAP
  • HCAPbamacan
  • SMC-3
  • SMC3L1CDLS3
  • structural maintenance of chromosomes 3

Background

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMC3
Uniprot