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SLPI Antibody (31)

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Western Blot: SLPI Antibody (31) [NBP1-42437] - 1. Recombinant SLPI 100 ng/ml, non-boiled 2. BAL (COPD) non boiled.
Western Blot: SLPI Antibody (31) [NBP1-42437] - 1. Recombinant SLPI 100 ng/ml, non-boiled 2. BAL (COPD) non boiled.
Immunohistochemistry-Frozen: SLPI Antibody (31) [NBP1-42437] - Human tonsil frozen sections

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clone
31
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

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SLPI Antibody (31) Summary

Immunogen
SLPI purified from sputum
Specificity
The antibody reacts specificly with secretory leukocyte proteinase inhibitor (SLPI; also known as antileukoprotease (ALP)).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLPI
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS containing 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein G purified

Alternate Names for SLPI Antibody (31)

  • ALK1
  • ALPProtease inhibitor WAP4
  • antileukoproteinase
  • BLPISeminal proteinase inhibitor
  • HUSI
  • HUSI-I
  • Mucus proteinase inhibitor
  • secretory leukocyte peptidase inhibitor
  • secretory leukocyte protease inhibitor (antileukoproteinase)
  • Secretory leukocyte protease inhibitor
  • SLPI
  • WAP four-disulfide core domain protein 4
  • WAP4HUSI-1
  • WFDC4MPI

Background

SLPI is a 11.7 kDa cationic inhibitor of neutrophil elastase and to a lesser extent of cathepsin G. It is locally produced by epithelial cells in the lung, skin and other organs and by Polymorphonuclear leukocytes (PMN) and (in mice) by macrophages. In addition to its proteinase inhibitory properties that may serve to protect against proteolytic injury, it was recently shown that SLPI also displays several other functions such as antimicrobial and anti-inflammatory activities. These appear to be independent of its ability to inhibit PMN serine proteinases. SLPI has also been demonstrated to display antibacterial and antifungal activity at concentrations in which SLPI is present in mucosal secretions including those of the lung. Another possible role for SLPI is inhibition of protein-disulphide isomerase that is considered essential for invasion of a cell by the Human Immunodeficiency Virus (HIV).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SLPI Antibody (31)
NBP1-42437
SLPI Antibody (31)
Species: Hu
Applications: WB, ELISA, IHC, IP

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Product General Protocols

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FAQs for SLPI Antibody (NBP1-42437). (Showing 1 - 1 of 1 FAQ).

  1. We would like to order SLPi antibody (NBP1-42437). This product is recommended for detection of SLPI of human and rat origin. Can you provide me the info about the antigen used for Ab production? Did you test this product in rat? Or it is only a prediction due to sequence homology between species? If so, can you give me some reference for it?
    • The immunogen for this antibody is SLPI purified from sputum. Rat cross reactivity is based on sequence homology.

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Bioinformatics

Gene Symbol SLPI