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SLC1A5 Antibody (3085B) [Unconjugated]

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SLC1A5 was detected in immersion fixed paraffin-embedded sections of human prostate using Rabbit Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11628) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
SLC1A5 was detected in immersion fixed paraffin-embedded sections of human prostate using Rabbit Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11628) at 3 µg/ml for 1 hour at room temperature followed by ...read more
SLC1A5 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Rabbit Anti-Human SLC1A5 Monoclonal Antibody (Catalog # MAB11628) at 3 µg/ml for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ICC/IF
Clone
3085B
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB11628-SP
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SLC1A5 Antibody (3085B) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic Peptide
Accession # Q9JKD8
Specificity
Detects a synthetic peptide specific for human SLC1A5 around amino acid 520 in Direct ELISA.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1-10 ug/mL
  • Immunohistochemistry 1-10 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Instructions
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLC1A5 Antibody (3085B) [Unconjugated]

  • AAAT
  • Alanine/serine/cysteine transporter 2
  • ASC amino acid transporter-2
  • ASCT2
  • ATB(0)
  • ATBO
  • baboon M7 virus receptor
  • M7V1
  • M7VS1
  • neutral amino acid transporter B
  • neutral amino acid transporter B(0)
  • R16
  • RD114 virus receptor
  • RD114/simian type D retrovirus receptor
  • RDR
  • RDRC
  • SLC1A5
  • Sodium-dependent neutral amino acid transporter type 2
  • solute carrier family 1 (neutral amino acid transporter), member 5
  • Solute carrier family 1 member 5

Background

SLC1A5 is a sodium dependent antiporter also known as ASCT2 (ASC amino acid Transporter2). It is a member of the solute carrier (SLC) superfamily of transporters and plays an important role in tumors. It is one of the 3 proteins, including SLC7A5 and SLC3A2, that are among the highest differentially expressed genes in activated lymphocytes and cancerous cells. Pharmacological inhibition of SLC1A5 was found to abrogate the effector functions of NK cells. In a pancancer analysis of SLC1A5, it has been demonstrated to be a prognostic biomarker in cancer patients.
  1. Nachef M, Ali AK, Almutairi SM, Lee SH. Targeting SLC1A5 and SLC3A2/SLC7A5 as a Potential Strategy to Strengthen Anti-Tumor Immunity in the Tumor Microenvironment. Front Immunol. 2021 Apr 19;12:624324. doi: 10.3389/fimmu.2021.624324. PMID: 33953707; PMCID: PMC8089370. 
  2. Chen P, Jiang Y, Liang J, Cai J, Zhuo Y, Fan H, Yuan R, Cheng S, Zhang Y. SLC1A5 is a novel biomarker associated with ferroptosis and the tumor microenvironment: a pancancer analysis. Aging (Albany NY). 2023 Aug 10;15(15):7451-7475. doi: 10.18632/aging.204911. Epub 2023 Aug 10. PMID: 37566748; PMCID: PMC10457057.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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