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Sirtuin 2/SIRT2 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76880AF350
Alexa Fluor 405
NBP1-76880AF405
Alexa Fluor 488
NBP1-76880AF488
Alexa Fluor 532
NBP1-76880AF532
Alexa Fluor 594
NBP1-76880AF594
Alexa Fluor 647
NBP1-76880AF647
Alexa Fluor 700
NBP1-76880AF700
Alexa Fluor 750
NBP1-76880AF750
Biotin
NBP1-76880B
DyLight 350
NBP1-76880UV
DyLight 405
NBP1-76880V
DyLight 488
NBP1-76880G
DyLight 550
NBP1-76880R
DyLight 594
NBP1-76880DL594
DyLight 650
NBP1-76880C
DyLight 680
NBP1-76880FR
DyLight 755
NBP1-76880IR
FITC
NBP1-76880F
HRP
NBP1-76880H
Janelia Fluor 525
NBP1-76880JF525
Janelia Fluor 549
NBP1-76880JF549
Janelia Fluor 585
NBP1-76880JF585
Janelia Fluor 635
NBP1-76880JF635
Janelia Fluor 646
NBP1-76880JF646
Janelia Fluor 669
NBP1-76880JF669
PerCP
NBP1-76880PCP
Unconjugated
NBP1-76880
mFluor Violet 450 SE
NBP1-76880MFV450
mFluor Violet 500 SE
NBP1-76880MFV500
mFluor Violet 610 SE
NBP1-76880MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-76880
BSA Free
NBP1-76880
BSA Free
NBP1-76880-0.025mg
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Sirtuin 2/SIRT2 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human SIRT2. The immunogen is located within the first 50 amino acids of SIRT2. Amino Acid Squence: AGKVQEAQDSDSDSEGG
Marker
Early Oligodendrocyte Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRT2
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Peptide affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Sirtuin 2/SIRT2 Antibody [CoraFluor™ 1]

  • EC 3.5.1
  • EC 3.5.1.-
  • FLJ35621
  • FLJ37491
  • S.cerevisiae, homolog) 2
  • SIR2L2
  • SIRT2
  • Sirtuin 2
  • sirtuin type 2

Background

Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast, Drosophila, and human. Also, endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase (2). Cytoskeleton-related protein, SIRT2 is down-regulated in gliomas, and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Sirtuin 2/SIRT2
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NBP1-76880CL1
Sirtuin 2/SIRT2 Antibody [CoraFluor™ 1]
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC

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Bioinformatics

Gene Symbol SIRT2