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Sirtuin 2/SIRT2 Antibody (4B11)

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Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of SIRT2 expression in transfected 293T cell line by SIRT2 monoclonal antibody (M01), clone 4B11.Lane 1: SIRT2 transfected lysate(39.5 KDa).Lane 2: Non-transfected lysate.
Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of SIRT2 expression in transfected 293T cell line by SIRT2 monoclonal antibody (M01), clone 4B11.Lane 1: SIRT2 transfected lysate(39.5 KDa).Lane ...read more
Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of monoclonal antibody to SIRT2 on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of SIRT2 over-expressed 293 cell line, cotransfected with SIRT2 Validated Chimera RNAi ( Cat # H00022933-R01V ) (Lane 2) or non-transfected ...read more
Sandwich ELISA: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Detection limit for recombinant GST tagged SIRT2 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, KD
Clone
4B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Sirtuin 2/SIRT2 Antibody (4B11) Summary

Immunogen
SIRT2 (AAH03012, 128 a.a. ~ 227 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSL
Localization
Cytoplasmic for short periods of time until complexes are formed (Sir2, Sir3 and Sir4). The complexes are recruited to their chromosome targets (nuclear) via interactions with DNA binding proteins, followed by deacetylation of histones H3 and H4. Therefor
Marker
Early Oligodendrocyte Marker
Specificity
SIRT2 - sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SIRT2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA and immunofluorescence.
Publications
Read Publication using H00022933-M01.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Sirtuin 2/SIRT2 Antibody (4B11)

  • EC 3.5.1
  • EC 3.5.1.-
  • FLJ35621
  • FLJ37491
  • S.cerevisiae, homolog) 2
  • SIR2L2
  • SIRT2
  • Sirtuin 2
  • sirtuin type 2

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Sirtuin 2/SIRT2
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H00022933-M01
Sirtuin 2/SIRT2 Antibody (4B11)
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Applications: WB, ELISA, ICC/IF, KD

Publications for Sirtuin 2/SIRT2 Antibody (H00022933-M01)(1)

We have publications tested in 1 confirmed species: Human.


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Bioinformatics

Gene Symbol SIRT2
Entrez
OMIM
Uniprot