![Western Blot: SHANK2 Antibody [NBP3-13215] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK2 antibody (NBP3-13215) diluted at 1:1000.](http://images.novusbio.com/fullsize/SHANK2-Antibody-Western-Blot-NBP3-13215-img0001.jpg "Western Blot: SHANK2 Antibody [NBP3-13215] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK2 antibody (NBP3-13215) diluted at 1:1000.")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of mouse SHANK2. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SHANK2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 159 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SHANK2 Antibody (NBP3-13215)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SHANK2 |
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK2 protein stained by SHANK2 antibody (NBP3-13215) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13215-img0003.jpg "Immunocytochemistry/Immunofluorescence: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK2 protein stained by SHANK2 antibody (NBP3-13215) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SHANK2 antibody (NBP3-13215) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SHANK2-Antibody-Immunohistochemistry-Paraffin-NBP3-13215-img0002.jpg "Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SHANK2 antibody (NBP3-13215) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")