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SHANK1 Antibody

Images

 
Western Blot: SHANK1 Antibody [NBP1-87016] - Analysis in human cell line U-2 OS and human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody [NBP1-87016] - Staining of human cell line U-2 OS shows localization to plasma membrane.
Immunohistochemistry-Paraffin: SHANK1 Antibody [NBP1-87016] - Staining of human lateral ventricle shows strong positivity in glial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SHANK1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TVKASIISELSSKLQQFGGSSAAGGALPWARGGSGGGGDSHHGGASYVPERTSSLQRQRLSDDSQSSLLSKPVSSLFQNWPKPPLP
Marker
TGN Marker
Predicted Species
Mouse (90%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SHANK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SHANK1 Protein (NBP1-87016PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SHANK1 Antibody

  • SH3 and multiple ankyrin repeat domains 1
  • SH3 and multiple ankyrin repeat domains protein 1
  • Shank1
  • Somatostatin receptor-interacting protein
  • SSTR-interacting protein
  • SSTRIPSPANK-1
  • synamon

Background

Shank is a novel family of postsynaptic density proteins (PSD) that bind to PSD-95-associated protein GKAP. Its putative function is to act as a scaffold protein in the PSD to cross-link NMDA receptors/PSD-95 complexes and couple them to regulators of the actin cytoskeleton. Shank1a is a splice variant of Shank1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SHANK1 Antibody (NBP1-87016) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SHANK1