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Recombinant Human SF2 His Protein

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SDS-Page: SF2 Protein [NBP2-53109]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

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Recombinant Human SF2 His Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-248 of Human SF2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRT

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SRSF1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
29.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human SF2 His Protein

  • Alternative-splicing factor 1
  • ASFpre-mRNA-splicing factor SF2, P33 subunit
  • MGC5228
  • serine/arginine-rich splicing factor 1
  • SF2FLJ53078
  • SF2p33
  • SFRS1splicing factor 2
  • Splicing factor, arginine/serine-rich 1ASF-1
  • SR splicing factor 1
  • SRp30a

Background

Serine/arginine-rich splicing factor 1, also known as SFRS1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Recombinant human SFRS1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SRSF1