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SETD7/9 Antibody (s4E5) - BSA Free

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Western Blot: SETD7/9 Antibody (s4E5) [NBP1-04298] - The Recombinant human SET7/9 protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were ...read more
Immunocytochemistry/ Immunofluorescence: SETD7/9 Antibody (s4E5) [NBP1-04298] - Analysis of SET7/9 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SET7/9 antibody (1:100) with ...read more
Flow Cytometry: SETD7/9 Antibody (s4E5) [NBP1-04298] - Analysis of SET7/9 in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. ...read more
Western Blot: SETD7/9 Antibody (s4E5) [NBP1-04298] - Recombinant human SET7/9 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed at a 1:2000. Proteins were visualized using a goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
s4E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

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SETD7/9 Antibody (s4E5) - BSA Free Summary

Immunogen
Recombinant human SETD7/9 (1-366aa) purified from E. coli
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SETD7
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:250-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for SETD7/9 Antibody (s4E5) - BSA Free

  • EC 2.1.1.43
  • H3-K4-HMTase SETD7
  • histone H3-lysine 4-specific methyltransferase
  • histone-lysine N-methyltransferase SETD7
  • KIAA1717SET domain-containing protein 7
  • KMT7FLJ21193
  • Lysine N-methyltransferase 7
  • SET domain containing (lysine methyltransferase) 7
  • SET7/9Histone H3-K4 methyltransferase SETD7
  • SET7Set9
  • SET9

Background

Set7/9 is a histone methyltransferase(HMTase) that transfers methyl group to Lys4 of histone H3, in complex with S-adenosyl-L-methionine(AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression.Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SETD7
Uniprot