![Western Blot: SerpinB2 Antibody [NBP1-33188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Western-Blot-NBP1-33188-img0008.jpg "Western Blot: SerpinB2 Antibody [NBP1-33188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SerpinB2. The exact sequence is proprietary. |
| Localization | Cytoplasm, Secreted, extracellular space |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SERPINB2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SerpinB2 Antibody (NBP1-33188)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SERPINB2 |
![Immunocytochemistry/Immunofluorescence: SerpinB2 Antibody [NBP1-33188] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by PAI2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33188-img0012.jpg "Immunocytochemistry/Immunofluorescence: SerpinB2 Antibody [NBP1-33188] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by PAI2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188] - NCIN87 xenograft , using PAI-2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Immunohistochemistry-Paraffin-NBP1-33188-img0004.jpg "Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188] - NCIN87 xenograft , using PAI-2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: SerpinB2 Antibody [NBP1-33188] - Sample (30 ug of whole cell lysate) JC 10% SDS PAGE; antibody diluted at 1:1000](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Western-Blot-NBP1-33188-img0002.jpg "Western Blot: SerpinB2 Antibody [NBP1-33188] - Sample (30 ug of whole cell lysate) JC 10% SDS PAGE; antibody diluted at 1:1000")
![Western Blot: SerpinB2 Antibody [NBP1-33188] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Western-Blot-NBP1-33188-img0006.jpg "Western Blot: SerpinB2 Antibody [NBP1-33188] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunoprecipitation: SerpinB2 Antibody [NBP1-33188] - Immunoprecipitation of PAI2 protein from U87-MG whole cell extracts using 5 ug of PAI2 antibody. Western blot analysis was performed using PAI2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Immunoprecipitation-NBP1-33188-img0010.jpg "Immunoprecipitation: SerpinB2 Antibody [NBP1-33188] - Immunoprecipitation of PAI2 protein from U87-MG whole cell extracts using 5 ug of PAI2 antibody. Western blot analysis was performed using PAI2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")