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Serpin A4/Kallistatin Antibody [Unconjugated]

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Western blot shows lysates of human liver tissue, human kidney tissue, and human pancreas tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Serpin A4/Kallistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1669) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Serpin A4/Kallistatin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human liver tissue, human kidney tissue, and human pancreas tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Serpin A4/Kallistatin Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1669
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1669-SP
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Serpin A4/Kallistatin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A4/Kallistatin
Gly20-Pro427 (predicted)
Accession # AAA59454
Specificity
Detects human Serpin A4/Kallistatin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A5, rhSerpin C1, recombinant mouse Serpin D1, and rhSerpin F2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SERPINA4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 10.8 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A4/Kallistatin Antibody [Unconjugated]

  • antitrypsin), member 4
  • KAL
  • Kallistatin
  • KLST
  • KSTKallikrein inhibitor
  • member 4
  • PI-4
  • PI4Peptidase inhibitor 4
  • protease inhibitor 4 (kallistatin)
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A4
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Serpin A4 is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as Kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
  3. Miao, R.Q. et al. (2002) Blood 100:3245.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SERPINA4
Uniprot