Home » Serpin A1/alpha 1-Antitrypsin » Serpin A1/alpha 1-Antitrypsin Antibodies » Serpin A1/alpha 1-Antitrypsin Antibody (1019625) [Unconjugated]

Serpin A1/alpha 1-Antitrypsin Antibody (1019625) [Unconjugated]

 
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Western blot shows lysates of human lung tissue, human kidney tissue, HT-29 human colon adenocarcinoma cell line, and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin A1/a1-Antitrypsin Monoclonal Antibody (Catalog # MAB12681) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human lung tissue, human kidney tissue, HT-29 human colon adenocarcinoma cell line, and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin ...read more
Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/a1‑Antitrypsin at approximately 65 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
1019625
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB12681-SP
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Serpin A1/alpha 1-Antitrypsin Antibody (1019625) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line, NS0-derived human Serpin A1/alpha 1-Antitrypsin
Glu25-Lys418
Accession # P01009
Specificity
Detects human SerpinA1/ alpha 1-Antitrypsin in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A1/alpha 1-Antitrypsin Antibody (1019625) [Unconjugated]

  • A1A
  • A1AT
  • AATMGC23330
  • alpha 1-Antitrypsin
  • alpha 1-Proteinase Inhibitor
  • Alpha-1 protease inhibitor
  • Alpha-1-antiproteinase
  • alpha-1-antitrypsin
  • antitrypsin), member 1
  • member 1
  • PIMGC9222
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • serine (or cysteine) proteinase inhibitor, clade A, member 1
  • Serpin A1
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe Serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring Serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe Serpin A1 deficiency (3).

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Barbour, K.W. et al. (2002) Genomics 80:515.
  3. Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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