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SENP2 Antibody (OTI3D3) - Azide and BSA Free

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Western Blot: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SENP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SENP2.
Western Blot: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SENP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SENP2.
Immunohistochemistry: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SENP2 mouse monoclonal antibody.
Flow Cytometry: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SENP2 ...read more
Western Blot: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-SENP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: ...read more
Immunohistochemistry: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SENP2 mouse monoclonal antibody.
Immunohistochemistry: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SENP2 mouse monoclonal antibody.
Immunohistochemistry: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SENP2 mouse monoclonal antibody.
Immunohistochemistry: SENP2 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74062] - Staining of paraffin-embedded Human Kidney tissue using anti-SENP2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI3D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74062AF350
Alexa Fluor 405
NBP2-74062AF405
Alexa Fluor 488
NBP2-74062AF488
Alexa Fluor 532
NBP2-74062AF532
Alexa Fluor 594
NBP2-74062AF594
Alexa Fluor 647
NBP2-74062AF647
Alexa Fluor 700
NBP2-74062AF700
Alexa Fluor 750
NBP2-74062AF750
Allophycocyanin
NBP2-74062APC
Biotin
NBP2-74062B
CoraFluor 1
NBP2-74062CL1
DyLight 350
NBP2-74062UV
DyLight 405
NBP2-74062V
DyLight 488
NBP2-74062G
DyLight 550
NBP2-74062R
DyLight 594
NBP2-74062DL594
DyLight 650
NBP2-74062C
DyLight 680
NBP2-74062FR
DyLight 755
NBP2-74062IR
FITC
NBP2-74062F
HRP
NBP2-74062H
Janelia Fluor 525
NBP2-74062JF525
Janelia Fluor 549
NBP2-74062JF549
Janelia Fluor 646
NBP2-74062JF646
Janelia Fluor 669
NBP2-74062JF669
PE
NBP2-74062PE
PerCP
NBP2-74062PCP
Unconjugated
NBP2-03852
mFluor Violet 450 SE
NBP2-74062MFV450
mFluor Violet 500 SE
NBP2-74062MFV500
mFluor Violet 610 SE
NBP2-74062MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-03852
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SENP2 Antibody (OTI3D3) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 139-523 of human SENP2 (NP_067640) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SENP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SENP2 Antibody (OTI3D3) - Azide and BSA Free

  • AXAM2
  • AXAM2Axam2
  • DKFZp762A2316
  • EC 3.4.22
  • EC 3.4.22.68
  • KIAA1331sentrin-specific protease 2
  • SENP2
  • Sentrin/SUMO-specific protease SENP2
  • SMT3IP2
  • SMT3IP2EC 3.4.22.-
  • SMT3-specific isopeptidase 2
  • SUMO1/sentrin/SMT3 specific peptidase 2
  • SUMO1/sentrin/SMT3 specific protease 2

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SENP2 Antibody
(OTI3D3) - Azide and ...
NBP2-74062
SENP2 Antibody (OTI3D3) - Azide and BSA Free
Species: Hu, Mu, Rt, Pm, Pm
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

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Bioinformatics

Gene Symbol SENP2