Genetic Strategies: Western Blot: SELS Antibody [NBP1-89558] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SELENOS antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: SELS Antibody [NBP1-89558] - Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558] - Staining of human tonsil shows strong cytoplasmic positivity in a small subset of germinal center cells.
Western Blot: SELS Antibody [NBP1-89558] - Analysis in human cell line HEK 293.
Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
This antibody was developed against Recombinant Protein corresponding to amino acids: EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKGNAKKPQEEDSPGPSTSSVLKRKSDRKPLRGGGYNPLSGEGGGACSWRPGR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VIMP
Purity
Immunogen affinity purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for SELS Antibody
ADO15
MGC104346
SBBI8
selenoprotein S
SelS
SEPS1
VCP-interacting membrane protein
VIMPMGC2553
Background
SELS encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Studies suggest that this protein may regulate cytokine production, and thus play a key role in the control of the inflammatory response. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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