Novus Biologicals products are now on bio-techne.com

Derlin 1 Antibody

Images

 
Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.
Immunocytochemistry/ Immunofluorescence: Derlin 1 Antibody [NB100-448] - Derlin-1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: Derlin 1 Antibody [NB100-448] - Staining of human liver.

Product Details

Summary
Reactivity Hu, Mu, Ca, ChHa, Rt, Bv, Ch, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Derlin 1 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of human Derlin 1 (between residues 200-251). [UniProt# Q9BUN8]
Localization
Endoplasmic reticulum
Predicted Species
Rat (100%), Chicken (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DERL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:2000
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 1:1000-1:5000
Application Notes
In Western blot, a band is seen at ~29 kDa. In ICC/IF, ER staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Derlin 1 Overexpression Lysate
Control Peptide
Derlin 1 Peptide (NB100-448PEP)
Publications
Read Publications using
NB100-448 in the following applications:

Reactivity Notes

Predicted to react with Xenopus, bovine, chicken, rat, zebrafish, and mouse based on 100% sequence homology. Mouse reactivity reported in scientific literature (PMID: 25377857)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Derlin 1 Antibody

  • DER-1
  • DER1Degradation in endoplasmic reticulum protein 1
  • Der1-like domain family, member 1
  • Der1-like protein 1
  • derlin-1
  • DERtrin-1
  • FLJ13784
  • FLJ42092
  • MGC3067
  • PRO2577

Background

Derlin 1 (degradation in endoplasmic reticulum protein 1) belongs to Derlin family and is a functional sub-unit of the ER-associated degradation (ERAD), a ubiquitin/proteasome dependent degradation process for proteins which fails to fold correctly or assemble into oligomeric complexes in ER. Derlin 1 forms a channel which permits the retrotranslocation of misfolded proteins into cytosol from ER wherein they undergo ubiquitination mediated proteasomal degradation. Derlin 1 can generate homo-/hetero-oligomers with DERL2 and DERL3, and interacts with several other proteins such as AMFR, VIMP/SELS, SEL1L, SYVN1 and VCP, as well as with SEL1L-SYVN1 and VCP-VIMP protein complexes. It also interacts with cytomegalovirus US11 protein, NGLY1 and YOD1, RNF103 etc. Expressed ubiquitously, it can get up-regulated in response to ER stress via ERN1-XBP1 pathway of the UPR/unfolded protein response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1558
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-Fr, IP, WB
NB100-1483
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, PEP-ELISA, PLA, WB
NB100-2526
Species: Ha, Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, KO, WB
NB110-40591
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-43772
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-85311
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-47485
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-89558
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-06274
Species: Ce, Ch, Dr, Hu, Mu, Rt, Sh, Ze
Applications: Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, Simple Western, WB
H00091319-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP2-93746
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
AF1543
Species: Hu
Applications: IHC, WB
NBP3-33259
Species: Hu, Mu, Rt
Applications: ELISA, WB
H00009695-Q01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP2-45970
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-31302
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB

Publications for Derlin 1 Antibody (NB100-448)(2)

Reviews for Derlin 1 Antibody (NB100-448) (0)

There are no reviews for Derlin 1 Antibody (NB100-448). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for Derlin 1 Antibody (NB100-448): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Derlin 1 Antibody (NB100-448). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in the product, NB100-448. Do you have any information about whether or not NB100-448 cross reacts with Drosophila after validation or based on sequence homology?
    • Our Derlin 1 antibody with catalogue number NB100-448 was raised to a synthetic peptide derived from the C-terminal region of human Derlin 1 (between residues 200-251). I have aligned the human and Drosophila sequences for you, and the sequence homology in this region is just 42%; as such NB100-448 is unlikely to recognise the Drosophila protein, although we do not have any testing data at present to confirm this. Human Drosophila. I am sorry for the inconvenience.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Derlin 1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol DERL1