![Immunocytochemistry/Immunofluorescence: SDHB Antibody [NB600-793] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/SDHB-Antibody-Immunocytochemistry-Immunofluorescence-NB600-793-img0003.jpg "Immunocytochemistry/Immunofluorescence: SDHB Antibody [NB600-793] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, Ctrl |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-ATYKEKKASV corresponding to C-Terminus according to NP_002991.2. |
| Marker | Mitochondria Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SDHB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western blot: Approx 28-30kDa band observed in lysates of cell lines HEK293, Jurkat and HeLa (calculated MW of 31.6kDa according to NP_002991.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB600-793 | Applications | Species |
|---|---|---|
| Selak MA, Armour SM, MacKenzie ED et al. Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase. Cancer Cell 2005-01-01 [PMID: 15652751] |
Secondary Antibodies |
Isotype Controls |
Research Areas for SDHB Antibody (NB600-793)Find related products by research area.
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HIF Antibodies: Beyond HIF-1 alpha The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the... Read full blog post. |
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| Gene Symbol | SDHB |
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![Immunohistochemistry: SDHB Antibody [NB600-793] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/SDHB-Antibody-Immunohistochemistry-NB600-793-img0005.jpg "Immunohistochemistry: SDHB Antibody [NB600-793] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Flow Cytometry: SDHB Antibody [NB600-793] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/SDHB-Antibody-Flow-Cytometry-NB600-793-img0002.jpg "Flow Cytometry: SDHB Antibody [NB600-793] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Control: SDHB Antibody [NB600-793] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/SDHB-Antibody-Control-NB600-793-img0004.jpg "Control: SDHB Antibody [NB600-793] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.")
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