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SATB1 Antibody (815427) [Unconjugated]

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SATB1 Monoclonal Antibody (Catalog # MAB8075) followed by HRP-conjugated Anti-Mouse ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for SATB1 at approximately 113 kDa (as indicated) using 10 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clone
815427
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
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SATB1 Antibody (815427) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SATB1
Met1-Ser260
Accession # Q01826
Specificity
Detects human and mouse SATB1 in ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SATB1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SATB1 Antibody (815427) [Unconjugated]

  • DNA-binding protein SATB1
  • SATB homeobox 1
  • SATB1
  • special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's)
  • Special AT-rich sequence-binding protein 1

Background

SATB1 (special AT-rich sequence binding protein-1) is an approximately 103 kDa nuclear matrix-associated homeobox transcription factor that binds AT rich DNA sequences at scaffolding protein attachment and viral genome integration sites. It is a key factor integrating higher-order chromatin architecture with gene regulation. Specifically, it is involved in MHC class-I gene organization and chromatin organization in undifferentiated thymocytes. SATB1 is cleaved into 65-70 kDa and 25‑30 kDa fragments during apoptosis. Human SATB1 shares 99% amino acid sequence identity with mouse and rat SATB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SATB1
Uniprot