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SAP30 Antibody (4D2)

Images

 
Immunocytochemistry/ Immunofluorescence: SAP30 Antibody (4D2) [H00008819-M02] - Analysis of monoclonal antibody to SAP30 on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SAP30 Antibody (4D2) [H00008819-M02] - Analysis of monoclonal antibody to SAP30 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 1.5 ug/ml.
ELISA: SAP30 Antibody (4D2) [H00008819-M02] - Detection limit for recombinant GST tagged SAP30 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
4D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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SAP30 Antibody (4D2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
SAP30 (NP_003855, 131 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDDDGGDSPVQDIDTPEVDLYQLQVNTLRRYKRHFKLPTRPGLNKAQLVEIVGCHFRSIPVNEKDTLTYFIYSVKNDKNKSDLKVDSGV
Specificity
SAP30 (4D2)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SAP30
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SAP30 Antibody (4D2)

  • 30 kDa Sin3-associated polypeptide
  • histone deacetylase complex subunit SAP30
  • Sin3 corepressor complex subunit SAP30
  • Sin3A-associated protein, 30kDa
  • Sin3-associated polypeptide p30
  • sin3-associated polypeptide, 30 kDa
  • Sin3-associated polypeptide, 30kDa

Background

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SAP30
Entrez
OMIM
Uniprot