Home » S100A9 » S100A9 Antibodies » S100A9 Antibody (OTI10D5) [Janelia Fluor® 646]

S100A9 Antibody (OTI10D5) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI10D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73970AF350
Alexa Fluor 405
NBP2-73970AF405
Alexa Fluor 488
NBP2-73970AF488
Alexa Fluor 532
NBP2-73970AF532
Alexa Fluor 594
NBP2-73970AF594
Alexa Fluor 647
NBP2-73970AF647
Alexa Fluor 700
NBP2-73970AF700
Alexa Fluor 750
NBP2-73970AF750
Biotin
NBP2-73970B
CoraFluor 1
NBP2-73970CL1
DyLight 350
NBP2-73970UV
DyLight 405
NBP2-73970V
DyLight 488
NBP2-73970G
DyLight 550
NBP2-73970R
DyLight 594
NBP2-73970DL594
DyLight 650
NBP2-73970C
DyLight 680
NBP2-73970FR
DyLight 755
NBP2-73970IR
FITC
NBP2-73970F
HRP
NBP2-73970H
Janelia Fluor 525
NBP2-73970JF525
Janelia Fluor 549
NBP2-73970JF549
Janelia Fluor 669
NBP2-73970JF669
Unconjugated
NBP2-45662
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45662
Azide and BSA Free
NBP2-73970
Novus Biologicals offers 100% Guarantee

S100A9 Antibody (OTI10D5) [Janelia Fluor® 646] Summary

Immunogen
Full length recombinant protein of human S100A9 (NP_002956) produced in SF9 cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
S100A9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for S100A9 Antibody (OTI10D5) [Janelia Fluor® 646]

  • CAGBMigration inhibitory factor-related protein 14
  • Calgranulin B
  • calgranulin-B
  • Calprotectin L1H subunit
  • CFAGMRP-14,60B8AG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP-14
  • MRP14Leukocyte L1 complex heavy chain
  • NIF
  • P14
  • protein S100-A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100 calcium binding protein A9
  • S100 calcium-binding protein A9 (calgranulin B)
  • S100 calcium-binding protein A9
  • S100A9

Background

S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 may function in the inhibition of casein kinase, and altered expression is associated with the disease cystic fibrosis. Overexpression of S100A9 has also recently been linked to a poor prognosis with invasive ductal carcinoma of the breast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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S100A9 Antibody
(OTI10D5) [Janelia Fl ...
NBP2-73970JF646
S100A9 Antibody (OTI10D5) [Janelia Fluor® 646]
Species: Hu
Applications: WB, IHC

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Product General Protocols

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FAQs for S100A9 Antibody (NBP2-73970JF646). (Showing 1 - 2 of 2 FAQ).

  1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol S100A9