![Immunohistochemistry-Paraffin: S100A8/A9 Antibody (MRP8 7C12/4) - Azide and BSA Free [NBP2-47667] - Human Tonsil stained with Macrophage L1 Protein Monoclonal Antibody (MAC387)](http://images.novusbio.com/fullsize/S100A8-A9-Antibody-MRP8-7C12-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47667-img0002.jpg "Immunohistochemistry-Paraffin: S100A8/A9 Antibody (MRP8 7C12/4) - Azide and BSA Free [NBP2-47667] - Human Tonsil stained with Macrophage L1 Protein Monoclonal Antibody (MAC387)")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Gt, Pm, Pm, RbSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | MAC387 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45295). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Affinity Purified monocyte membrane preparation |
| Localization | Cytoplasmic |
| Specificity | Recognizes the L1 or Calprotectin molecule (S100A9), an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. This monoclonal antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | S100A9 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for S100A8/A9 Antibody (NBP2-47667)Find related products by research area.
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| Gene Symbol | S100A9 |
![Flow Cytometry: S100A8/A9 Antibody (MRP8 7C12/4) - Azide and BSA Free [NBP2-47667] - Mouse splenocytes were fixed and permeabilized with Foxp3 Fixation/Permeabilization kit. Splenocytes were incubated with Fc block for 5 mins at RT and stained with or without antibody at 1:100 dilution for 25 mins at RT. Data was acquired on a Cytoflex LX and analyzed using FlowJo 10. This image was added from customer review. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/S100A8-A9-Antibody-MRP8-7C12-4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47667-img0001.jpg "Flow Cytometry: S100A8/A9 Antibody (MRP8 7C12/4) - Azide and BSA Free [NBP2-47667] - Mouse splenocytes were fixed and permeabilized with Foxp3 Fixation/Permeabilization kit. Splenocytes were incubated with Fc block for 5 mins at RT and stained with or without antibody at 1:100 dilution for 25 mins at RT. Data was acquired on a Cytoflex LX and analyzed using FlowJo 10. This image was added from customer review. Image from the Alexa Fluor 488 version of this antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")