RUNX2/CBFA1 Antibody - BSA Free

Runt-related transcription factor 2 (RUNX2), also known as CBFA1, AML-3, PEBP-2alphaA, and OSF-2, is a transcription factor that places a critical role in osteoblast differentiation and bone development (1-3). RUNX2 is a DNA-binding protein that belongs to the RUNX family which share a common runt domain (3). RUNX2 has two main isoforms which vary based on the two promoter regions (3). The main canonical isoform (P1) has MASN/DS at its N-terminus while the other (P2) isoform includes a MRIPV pentapeptide at its N-terminus (3). The RUNX2 P1 isoform has a theoretical molecular weight of 56 kDa and is synthesized as a 521 amino acid (aa) protein containing multiple domains. Specifically, RUNX2 contains transactivation domains (AD1, 2 and 3), a glutamine/alanine (Q/A)-rich domain, a runt homology domain (RHD), a nuclear localization signal (NLS), a proline/serine/threonine (PST)-rich domain, a nuclear matrix targeting signal (NMTS), a repression domain (RD), and a VWRPY region (3). RUNX2 is a heterodimer of an alpha and beta subunit where the alpha subunit binds DNA through the runt domain and the binding affinity is increased through heterodimerization (4).

Functionally, RUNX2 promotes the expression of osteoblast-specific genes vital for the osteoblast differentiation and proliferation process including type I collagen, osteocalcin (OCN), and alkaline phosphatase (APC) (1, 3). Further evidence for the role of RUNX2 is highlighted by a study of Runx2-/-mice which completely lack osteoblasts (4). Additionally, RUNX2 is also required for chondrocyte maturation, which are the cells responsible for cartilage formation (1, 3, 5). Given the role of RUNX2 in bone and cartilage maturation and formation, it is clear that defects or mutations in RUNX2 cause various bone and bone-related diseases (3, 6, 7). For instance, cleidocranial dysplasia (CCD), which presents with delayed cranial suture closure phenotypes, hypoplastic clavicles, extra teeth, and short stature, is caused by haploinsufficiency in RUNX2 (2, 3, 6). Furthermore, metaphyseal dysplasia with maxillary hypoplasia and brachydactyly (MDMHB) is a bone dysplasia disorder with a phenotype of abnormalities in the long bones, an underdeveloped jawbone, and short fingers that is caused by a duplication in RUNX2 (6). Finally, RUNX2 has been shown to be upregulated in mouse models of the joint disorder osteoarthritis (OA) and may be a potential molecular target for disease treatment (7).

Alternative names for RUNX2 include Acute myeloid leukemia 3 protein CBFA1, CBF-alpha-1, CCD1, CCDAML3, CLCD, Core-binding factor subunit alpha-1, MGC120023, ML3, oncogene AML-3, OSF2, osteoblast-specific transcription factor 2, PEA2aA, PEA2-alpha A, PEBP2A, polyomavirus enhancer-binding protein 2 alpha A subunit, runt related transcription factor 2, SL3/AKV core-binding factor alpha A subunit, and SL3-3 enhancer factor 1 alpha A subunit.

References

1. Ferreira, L. B., Gimba, E., Vinagre, J., Sobrinho-Simoes, M., & Soares, P. (2020). Molecular Aspects of Thyroid Calcification. International journal of molecular sciences. https://doi.org/10.3390/ijms21207718

2. Kim, W. J., Shin, H. L., Kim, B. S., Kim, H. J., & Ryoo, H. M. (2020). RUNX2-modifying enzymes: therapeutic targets for bone diseases. Experimental & molecular medicine. https://doi.org/10.1038/s12276-020-0471-4

3. Vimalraj, S., Arumugam, B., Miranda, P. J., & Selvamurugan, N. (2015). Runx2: Structure, function, and phosphorylation in osteoblast differentiation. International journal of biological macromolecules. https://doi.org/10.1016/j.ijbiomac.2015.04.008

4. Uniprot (Q13950)

5. Komori T. (2017). Roles of Runx2 in Skeletal Development. Advances in experimental medicine and biology. https://doi.org/10.1007/978-981-10-3233-2_6

6. Moffatt, P., Ben Amor, M., Glorieux, F. H., Roschger, P., Klaushofer, K., Schwartzentruber, J. A., Paterson, A. D., Hu, P., Marshall, C., FORGE Canada Consortium, Fahiminiya, S., Majewski, J., Beaulieu, C. L., Boycott, K. M., & Rauch, F. (2013). Metaphyseal dysplasia with maxillary hypoplasia and brachydactyly is caused by a duplication in RUNX2. American journal of human genetics. https://doi.org/10.1016/j.ajhg.2012.12.001

7. Chen, D., Kim, D. J., Shen, J., Zou, Z., & O'Keefe, R. J. (2019). Runx2 plays a central role in Osteoarthritis development. Journal of orthopaedic translation. https://doi.org/10.1016/j.jot.2019.11.008

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-P, Immunocytochemistry/ Immunofluorescence, In vivo assay, WB.

Showing Publications 1 - 10 of 17. Show All 17 Publications. Collapse Publications.

Publications using NBP1-77461	Applications	Species
Sousa MGDC, Balbinot GS, Subbiah R et al. In vitro development and optimization of cell-laden injectable bioprinted gelatin methacryloyl (GelMA) microgels mineralized on the nanoscale bioRxiv : the preprint server for biology 2023-10-12 [PMID: 37873385] (Immunocytochemistry/ Immunofluorescence, Mouse)	Immunocytochemistry/ Immunofluorescence	Mouse
Chan, B;Glogauer, M;Wang, Y;Wrana, J;Chan, K;Beier, F;Bali, S;Hinz, B;Parreno, J;Ashraf, S;Kandel, R; Adseverin, an actin-binding protein, modulates hypertrophic chondrocyte differentiation and osteoarthritis progression Science advances 2023-08-04 [PMID: 37540756] (In vivo assay)	In vivo assay
Poudel SB, Ruff RR, Yildirim G et al. Excess growth hormone triggers inflammation-associated arthropathy, subchondral bone loss, and arthralgia The American journal of pathology 2023-03-02 [PMID: 36870529] (IHC-P, Mouse)	IHC-P	Mouse
Subbiah R, Lin EY, Athirasala A et al. Engineering of an osteoinductive and growth factor-free injectable bone-like microgel for bone regeneration Advanced healthcare materials 2023-02-20 [PMID: 36808718]
Zanut A, Li R, Deng R et al. A polymer canvas with the stiffness of the bone matrix to study and control mesenchymal stem cell response Advanced healthcare materials 2022-12-24 [PMID: 36565136]
Ciavarella C, Motta I, Vasuri F et al. Involvement of miR-30a-5p and miR-30d in Endothelial to Mesenchymal Transition and Early Osteogenic Commitment under Inflammatory Stress in HUVEC Biomolecules 2021-02-05 [PMID: 33562690] (ICC/IF, Human)	ICC/IF	Human
Serjeant M, Moon PM, Quinonez D, et al. The Role of Panx3 in Age-Associated and Injury-Induced Intervertebral Disc Degeneration International journal of molecular sciences 2021-01-22 [PMID: 33499145] (IF/IHC, Mouse)	IF/IHC	Mouse
Cahill SV, Kwon HK, Back J et al. Locally-delivered Adjuvant Biofilm-penetrating Antibiotics Rescue Impaired Endochondral Fracture Healing Caused by MRSA Infection Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2020-12-18 [PMID: 33336805]
Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 2020-04-02 [PMID: 32243809] (Mouse)		Mouse
Darrieutort-Laffite C, Arnolfo P, Garraud T et al. Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner J Clin Med [PMID: 31561454] (IF/IHC)	IF/IHC
Thrivikraman G, Athirasala A, Gordon R et al. Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization Nat Commun 2019-08-06 [PMID: 31388010] (ICC/IF, Human)	ICC/IF	Human
Priante G, Ceol M, Gianesello L et al. Human proximal tubular cells can form calcium phosphate deposits in osteogenic culture: role of cell death and osteoblast-like transdifferentiation. Cell Death Discov 2019-01-28 [PMID: 30701089] (WB, Human)	WB	Human
Priante G, Quaggio F, Gianesello L et al. Caspase-independent programmed cell death triggers Ca2PO4 deposition in an in vitro model of nephrocalcinosis. Biosci. Rep. 2017-12-05 [PMID: 29208768] (WB, Human)	WB	Human
Pillai IC, Li S, Romay M et al. Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification. Cell Stem Cell. 2016-11-15 [PMID: 27867037]
Mao AS, Shin JW, Utech S et al. Deterministic encapsulation of single cells in thin tunable microgels for niche modelling and therapeutic delivery. Nat Mater. 2016-10-31 [PMID: 27798621] Details: This citation used the Alexa Fluor 647 version of this antibody.
Mao AS, Shin JW, Mooney DJ. Effects of substrate stiffness and cell-cell contact on mesenchymal stem cell differentiation. Biomaterials. 2016-05-05 [PMID: 27203745] (ICC/IF, Mouse) Details: This publication used the AF647 conjugated form of this antibody (NBP1-77461AF647)	ICC/IF	Mouse
Al-Rekabi Z, Wheeler MM, Leonard A et al. Activation of the IGF1 Pathway Mediates Changes in Cellular Contractility and Motility in Single-Suture Craniosynostosis J Cell Sci 2015-12-11 [PMID: 26659664] (ICC/IF, Human) Details: This citation used the DyLight 488 version of this antibody.	ICC/IF	Human
Show All 17 Publications. Collapse Publications.

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• RUNX2/CBFA1 Antibodies
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• RUNX2/CBFA1 Lysates
• RUNX2/CBFA1 Proteins