![Western Blot: RUFY1 Antibody [NBP1-68865] - NBP1-68865 (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RUFY1-Antibody-Western-Blot-NBP1-68865-img0002.jpg "Western Blot: RUFY1 Antibody [NBP1-68865] - NBP1-68865 (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-QKNEAITSFEGKT corresponding to internal region according to NP_001035541.1. |
| Epitope | C-QKNEAITSFEGKT |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | RUFY1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 79.8kDa according to NP_079434.3). Primary incubation was 1 hour IHC: Paraffin embedded Human Small Intestine. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-68865 | Applications | Species |
|---|---|---|
| Matsuoka S, Ballif BA, Smogorzewska A et al. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 2007-05-25 [PMID: 17525332] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RUFY1 |
| Entrez | |
| Uniprot |
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![Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP1-68865] - 5ug/ml staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize/RUFY1-Antibody-Immunohistochemistry-Paraffin-NBP1-68865-img0003.jpg "Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP1-68865] - 5ug/ml staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.")
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