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RPS20 Antibody

Images

 
Western Blot: RPS20 Antibody [NBP1-80804] - Analysis in human cell line A-549.
Immunocytochemistry/ Immunofluorescence: RPS20 Antibody [NBP1-80804] - Staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804] - Staining of human lymph node shows moderate cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: RPS20 Antibody [NBP1-80804] - Staining of human fallopian tube shows moderate positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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RPS20 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIA
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS20
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
RPS20 Protein (NBP1-80804PEP)
Publications
Read Publications using
NBP1-80804 in the following applications:

  • WB
    3 publications

Reactivity Notes

Reactivity reported in scientific literature (PMID: 25866880).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPS20 Antibody

  • 40S ribosomal protein S20
  • FLJ27451
  • MGC102930
  • ribosomal protein S20

Background

Mammalian ribosomal proteins are encoded by multigene families that consist of processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S20, also known as RPS20, is a 119 amino acid cytoplasmic protein that is a component of the 40S ribosomal subunit. Co-transcribed with the small nucleolar RNA gene U54, Ribosomal Protein S20 is a primary binding protein (it binds independently to its target protein) that interacts with both the 5' and 3' minor domains of 16S ribosomal RNA (rRNA). Through its interactions with 16S rRNA, Ribosomal Protein S20 is thought to play a key role in nucleating the assembly of the 30S ribosomal subunit. Like most ribosomal protein-coding genes, the gene encoding Ribosomal Protein S20 is dispersed throughout the genome and exists as multiple processed pseudogenes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPS20 Antibody (NBP1-80804)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-80804 Applications Species
Arpit Dheeraj, Fernando Jose Garcia Marques, Dhanir Tailor, Abel Bermudez, Angel Resendez, Mallesh Pandrala, Benedikt Grau, Praveen Kumar, Carrsyn B. Haley, Alexander Honkala, Praveen Kujur, Stefanie S. Jeffrey, Sharon Pitteri, Sanjay V. Malhotra Inhibition of protein translational machinery in triple-negative breast cancer as a promising therapeutic strategy Cell Reports Medicine 2024-05-09 [PMID: 38729158]
Sawamoto A, Doi K, Amakura Y et al. N-Caffeoyltryptophan enhances adipogenic differentiation in preadipocytes and improves glucose tolerance in mice Biochimica et Biophysica Acta (BBA) - General Subjects 2022-11-01 [PMID: 36460233] (WB, Mouse)

Details:
1:1000 dilution WB
WB Mouse
Lin J, Lai X, Fan X et al. Oridonin Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting GSDMD-Mediated Pyroptosis Genes 2022-11-17 [PMID: 36421808] (WB, Mouse)

Details:
Dilutions: 1:1000
WB Mouse
Park J, Lee J, Kim JH et al. ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD Nucleic acids research 2021-09-22 [PMID: 34551427] (WB, Human) WB Human
Zhang X, Gao X, Coots RA et al. Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. Nat Struct Mol Biol 2015-05-01 [PMID: 25866880]
Stadler C, Hjelmare M, Neumann B et al. Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy. J Proteomics 2012-04-03 [PMID: 22361696]
Badhai J, Frojmark AS, Razzaghian HR et al. Posttranscriptional down regulation of small ribosomal subunit proteins correlates with reduction of 18S rRNA in RPS19 deficiency. FEBS Lett 2009-06-18 [PMID: 19454283]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPS20 Antibody (NBP1-80804) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPS20
Entrez
Uniprot