![Western Blot: RPS18 Antibody [NBP2-93632] - Western blot analysis of extracts of various cell lines, using RPS18 antibody (NBP2-93632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/RPS18 Antibody-Western Blot-NBP2-93632-img0005.jpg "Western Blot: RPS18 Antibody [NBP2-93632] - Western blot analysis of extracts of various cell lines, using RPS18 antibody (NBP2-93632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human RPS18 (NP_072045.1). MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RPS18 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.05% Proclin 300 |
| Purity | Affinity purified |
| Publication using NBP2-93632 | Applications | Species |
|---|---|---|
| Lafita-Navarro MC, Hao YH, Jiang C et al. ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells Cell reports 2023-10-31 [PMID: 37851577] (Western Blot, Human) | Western Blot | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for RPS18 Antibody (NBP2-93632)Find related products by research area.
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| Gene Symbol | RPS18 |
![Immunocytochemistry/Immunofluorescence: RPS18 Antibody [NBP2-93632] - Analysis of PC-12 cells using RPS18 Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPS18-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93632-img0008.jpg "Immunocytochemistry/Immunofluorescence: RPS18 Antibody [NBP2-93632] - Analysis of PC-12 cells using RPS18 Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: RPS18 Antibody [NBP2-93632] - Rat brain using RPS18 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/RPS18-Antibody-Immunohistochemistry-Paraffin-NBP2-93632-img0004.jpg "Immunohistochemistry-Paraffin: RPS18 Antibody [NBP2-93632] - Rat brain using RPS18 antibody at dilution of 1:100 (40x lens).")
![Immunocytochemistry/Immunofluorescence: RPS18 Antibody [NBP2-93632] - Analysis of HepG2 cells using RPS18 Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPS18-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93632-img0006.jpg "Immunocytochemistry/Immunofluorescence: RPS18 Antibody [NBP2-93632] - Analysis of HepG2 cells using RPS18 Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: RPS18 Antibody [NBP2-93632] - Analysis of NIH/3T3 cells using RPS18 Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPS18-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93632-img0007.jpg "Immunocytochemistry/Immunofluorescence: RPS18 Antibody [NBP2-93632] - Analysis of NIH/3T3 cells using RPS18 Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: RPS18 Antibody [NBP2-93632] - Human thyroid cancer using RPS18 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/RPS18-Antibody-Immunohistochemistry-Paraffin-NBP2-93632-img0002.jpg "Immunohistochemistry-Paraffin: RPS18 Antibody [NBP2-93632] - Human thyroid cancer using RPS18 antibody at dilution of 1:100 (40x lens).")
![Immunohistochemistry-Paraffin: RPS18 Antibody [NBP2-93632] - Mouse kidney using RPS18 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/RPS18-Antibody-Immunohistochemistry-Paraffin-NBP2-93632-img0003.jpg "Immunohistochemistry-Paraffin: RPS18 Antibody [NBP2-93632] - Mouse kidney using RPS18 antibody at dilution of 1:100 (40x lens).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")