ROBO1 Antibody Summary
Description |
Stable for at least 1 year at -20°C. |
Immunogen |
Robo1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human Robo1. This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family members. [UniProt# Q9Y6N7] |
Specificity |
The antibody detects a 180 kDa band corresponding to Robo1 in Western blots of human HepG2 and HUVEC cells, as well as mouse C2C12 cells. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
ROBO1 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:200
- Western Blot 1:500
|
Theoretical MW |
180 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20C. |
Buffer |
PBS, 1 mg/ml BSA and 50% glycerol |
Preservative |
0.05% Sodium Azide |
Purity |
Antigen Affinity-purified |
Alternate Names for ROBO1 Antibody
Background
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. In Drosophila, the roundabout gene, a member of the immunoglobulin gene superfamily, encodes an integral membrane protein that is both an axon guidance receptor and a cell adhesion receptor. This receptor is involved in the decision by axons to cross the central nervous system midline. The protein encoded by this gene is structurally similar to the Drosophila roundabout protein. Two transcript variants encoding different isoforms have been found for this gene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Secondary Antibodies
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