ROBO1 Antibodies
We offer ROBO1 Antibodies for use in common research applications: ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each ROBO1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ROBO1 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Rat. Use the list below to choose the ROBO1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ROBO1 polyclonal antibodies and browse our ROBO1 monoclonal antibody catalog.
Alternate Names for ROBO1 Antibodies
anti-ROBO1 antibody, anti-DUTT1Drosophila) homolog 1 antibody, anti-H-Robo-1 antibody, anti-MGC131599 antibody, anti-MGC133277 antibody, anti-roundabout, axon guidance receptor, homolog 1 (Drosophila) antibody
15 Results for "robo1" in Primary Antibodies
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0015.jpg "Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.")
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NB600-1254] - Staining of Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1254-img0005.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - Affinity Purified ROBO1 antibody was used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0016.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Biotinylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0012.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western blot using Affinity Purified ROBO1 antibody shows detection of a band at ~181 kDa corresponding to ROBO1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0013.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: MCF-7 Lysate. Lane 4: Jurkat Lysate. Lane 5: A431 Lysate. Lane 6: A549 Lysate. Lane 7: LNCap Lysate. Lane 8: MOLT-4 Lysate. Lane 9: Ramos Lysate. Lane 10: Raji Lysate. Lane 11: A-172 Lysate. Lane 12: NIH/3T3 Lysate. Load: 35 ug per lane. Primary antibody: ROBO1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted/Observed size: 181kDa for ROBO1.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0014.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4C.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0011.jpg)
![ELISA: ROBO1 Antibody [NB600-1254] - ELISA results of purified ROBO1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/ROBO1-Antibody-ELISA-NB600-1254-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP3-23373] - Immunocytochemical labeling of ROBO1 in mouse C2C12. The cells were labeled with rabbit polyclonal ROBO1 (C-terminal region) in the absence or presence of blocking peptide. The antibody was then detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23373_rabbit-robo1-pab-19920231856944.jpg "Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP3-23373] - Immunocytochemical labeling of ROBO1 in mouse C2C12. The cells were labeled with rabbit polyclonal ROBO1 (C-terminal region) in the absence or presence of blocking peptide. The antibody was then detected using appropriate secondary antibodies conjugated to Cy3.")
![Western Blot: ROBO1 Antibody [NBP3-23373] - Western blot analysis of HepG2 (lanes 1 & 4), C2C12 (lanes 2 & 5), and HUVEC (lanes 3 & 6). The blot was probed with anti-ROBO1 (C-terminal region) in absence (lanes 1-3) or presence of ROBO1 (C-terminal region) blocking peptide (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23373_rabbit-robo1-pab-199202317594418.jpg)
![Western Blot: ROBO1 Antibody (10E2-R) [NBP3-32913] - Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (NBP3-32913) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 ug/Lane) Lane 2: NIH/3T3 cell lysate (10 ug/Lane) Lane 3: Rat brain tissue lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: RAW264.7 cell lysate (10 ug/Lane) Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32913) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32913_mouse-robo1-mab-10e2-r-288202412442967.jpg "Western Blot: ROBO1 Antibody (10E2-R) [NBP3-32913] - Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (NBP3-32913) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 ug/Lane) Lane 2: NIH/3T3 cell lysate (10 ug/Lane) Lane 3: Rat brain tissue lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: RAW264.7 cell lysate (10 ug/Lane) Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32913) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ROBO1 Antibody (10E2-R) [NBP3-32913] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-ROBO1 antibody (NBP3-32913) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32913) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32913_mouse-robo1-mab-10e2-r-29820241416189.jpg)
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP2-55652] - Staining of human cell line HeLa shows localization to plasma membrane.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55652-img0001.jpg "Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP2-55652] - Staining of human cell line HeLa shows localization to plasma membrane.")
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP2-68981] - Staining of human cell line HeLa shows localization to plasma membrane.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68981-img0001.jpg "Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP2-68981] - Staining of human cell line HeLa shows localization to plasma membrane.")