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ROBO1 Antibody (10E2-R)

Images

 
Western Blot: ROBO1 Antibody (10E2-R) [NBP3-32913] - Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (NBP3-32913) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 ug/Lane) Lane 2: ...read more
Immunohistochemistry: ROBO1 Antibody (10E2-R) [NBP3-32913] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-ROBO1 antibody (NBP3-32913) at 1/600 dilution. The section was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
10E2-R
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ROBO1 Antibody (10E2-R) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein within Human ROBO1 aa 51-300 / 1,651. (Uniprot: Q9Y6N7)
Localization
Cell membrane, Cell projection, axon, Endoplasmic reticulum-Golgi intermediate compartment membrane.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ROBO1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:600
  • Western Blot 1:1000
Theoretical MW
181 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for ROBO1 Antibody (10E2-R)

  • DUTT1
  • DUTT1Drosophila) homolog 1
  • H-Robo-1
  • MGC131599
  • MGC133277
  • ROBO1
  • roundabout, axon guidance receptor, homolog 1 (Drosophila)

Background

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. In Drosophila, the roundabout gene, a member of the immunoglobulin gene superfamily, encodes an integral membrane protein that is both an axon guidance receptor and a cell adhesion receptor. This receptor is involved in the decision by axons to cross the central nervous system midline. The protein encoded by this gene is structurally similar to the Drosophila roundabout protein. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ROBO1