![Western Blot: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Azide-and-BSA-Free-Western-Blot-NBP2-80925-img0001.jpg "Western Blot: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 7B3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human RICTOR expressed in E. coli. [UniProt# Q6R327] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RICTOR |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This RICTOR (7B3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. The observed molecular weight of the protein in WB may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 192 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Ammonium sulfate precipitation |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Rictor Antibody (NBP2-80925)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RICTOR |
![Immunocytochemistry/Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80925-img0002.jpg "Immunocytochemistry/Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80925-img0003.jpg "Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining. Image from the standard format of this antibody.")
![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0007.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.")
![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0004.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.")
![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0005.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.")
![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - An intracellular stain was performed on Jurkat cells with Rictor Antibody (7B3) NBP1-51645AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0006.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - An intracellular stain was performed on Jurkat cells with Rictor Antibody (7B3) NBP1-51645AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0008.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")