![Western Blot: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/mL mouse anti-RelA/NFkB p65 monoclonal (NB100-56712, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018). Image using the standard format of this product.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Azide-Free-Western-Blot-NBP2-27416-img0012.jpg "Western Blot: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/mL mouse anti-RelA/NFkB p65 monoclonal (NB100-56712, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018). Image using the standard format of this product.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, Flow, IHC, CyTOF-ready |
| Clone | 112A1021 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | This NfkB p65 antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RELA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10-15 uL of the recommended dilution is used per data point. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 ml sterile PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for RelA/NFkB p65 Antibody (NBP2-27416)Find related products by research area.
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Killing two birds with one stone: Treating inflammation and cancer by inhibiting prolyl-4-hydroxylase-1 By Jamshed Arslan Pharm.D. The cell’s oxygen-sensing machinery comprises prolyl-4-hydroxylases (P4Hs 1-3, PHDs 1-3, or EGLN 1-3) and their canonical target hypoxia-inducible factors (HIFs). When oxygen levels ... Read full blog post. |
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The effect of antioxidants and the NFkB p65 pathway in inflammation NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit... Read full blog post. |
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![Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Azide-Free-Immunohistochemistry-Paraffin-NBP2-27416-img0007.jpg "Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.")
![Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Azide-Free-Flow-Cytometry-NBP2-27416-img0004.jpg "Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.")
![Western Blot: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - analysis of p65 using p65 antibody at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Azide-Free-Western-Blot-NBP2-27416-img0006.jpg "Western Blot: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - analysis of p65 using p65 antibody at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.")
![Simple Western: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Azide-Free-Simple-Western-NBP2-27416-img0010.jpg "Simple Western: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Simple Western: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Detection of p65 by Simple Western (JESS) in chemiluminescence in nuclear extract of reconstructed human epidermis lysate (about 600 ug/mL protein concentration). Antibody at 1:20. Simple Western image submitted by a verified customer review. Image using the standard format of this product.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Azide-Free-Simple-Western-NBP2-27416-img0011.jpg "Simple Western: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Detection of p65 by Simple Western (JESS) in chemiluminescence in nuclear extract of reconstructed human epidermis lysate (about 600 ug/mL protein concentration). Antibody at 1:20. Simple Western image submitted by a verified customer review. Image using the standard format of this product.")
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