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Recombinant Mouse Wnt-16b Protein, CF

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RecombinantMouse Wnt-16b (Catalog # 9148-WN/CF) induces differentiation of the C3H10T1/2 mouse mesenchymal stem cell line to alkaline phosphatase secreting osteoblast cells. The ED50 for this effect is2-12 µg/mL.
RecombinantMouse Wnt-16b (Catalog # 9148-WN/CF) induces differentiation of the C3H10T1/2 mouse mesenchymal stem cell line to alkaline phosphatase secreting osteoblast cells. The ED50 for this effect is2-12 µg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
9148-WN-025
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Recombinant Mouse Wnt-16b Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 2-12 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-16b protein
Asn30-Lys364
Accession #
N-terminal Sequence
Asn30
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
43-63 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-16b Protein, CF

  • AHCP
  • WNT16
  • Wnt16b
  • Wnt-16b

Background

Wnt-16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine-rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mature mouse Wnt‑16 shares 92% and 97% aa sequence identity with human and rat Wnt‑16, respectively. Wnt-16 is expressed by uterine stromal cells adjacent to the luminal epithelium during implantation (4). It is
up-regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt-16 in mouse embryos and human amniotic fluid (6). It is secreted by osteoblasts and inhibits monocyte differentiation into osteoclasts, thereby contributing to cortical bone thickness and bone mineral density [1279, 7]. Wnt-16 is over-expressed in cells undergoing replicative senescence, and it is up-regulated in articular cartilage by injury and osteoarthritis (9, 10). Wnt-16b expression in skin is up-regulated and enhances cell survival in human basal cell carcinomas (11). Its expression is also up-regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (12). Pre-B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A-Pbx1 fusion protein, also causes up-regulation of Wnt-16 that confers resistance to apoptosis (13, 14). Wnt-16 signaling through both canonical and JNK-mediated (non-canonical) pathways is reported (9-11).
  1. Clevers, H. and R. Nusse (2012) Cell 149:1192.
  2. Katoh, H. and M. Katoh (2005) Oncol. Rep. 13:771.
  3. Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
  4. Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
  5. Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
  6. Nath, A.K. et al. (2009) PLoS One 4:e4221.
  7. Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
  8. Moverare-Skrtic, S. et al. (2014) Nat. Med. 20:1279.
  9. Dell'accio, F. et al. (2008) Arthritis Rheum. 58:1410.
  10. Binet, R. et al. (2009) Cancer Res. 69:9183.
  11. Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
  12. Sun, Y. et al. (2012) Nat. Med. 18:1359.
  13. McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
  14. Mazieres, J. et al. (2005) Oncogene 24:5396.

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Recombinant Mouse
Wnt-16b Protein, CF
9148-WN/CF
Recombinant Mouse Wnt-16b Protein, CF
Species: Mu
Applications: Bioactivity

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