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Recombinant Mouse Tie-2 Fc Chimera Protein, CF

 (6 Publications)
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Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Mouse Tie-2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can bind rhAngiopoietin-2 with a linear range of 2‑50 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Tie-2 protein
Mouse Tie-2
(Ala23 - Lys744)
Accession # CAA47857		 DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala23
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Tek
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
107.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
125-135 kDa, reducing conditions
Publications
Read Publications using
762-T2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Tie-2 Fc Chimera Protein, CF

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • Tie-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Background

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.

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Publications for Tie-2 (762-T2)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Binding Assay, Bioassay, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(3)
In Vivo
(2)
All Applications
Filter By Species
Mouse
(4)
All Species
Showing Publications 1 - 6 of 6.
Publications using 762-T2 Applications Species
I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk Nature Neuroscience, 2021-01-28;0(0):. 2021-01-28 [PMID: 33510480] (Bioassay, Mouse) Bioassay Mouse
C Helaine, AE Ferré, MM Leblond, EA Pérès, M Bernaudin, S Valable, E Petit Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study Cancers, 2020-11-30;12(12):. 2020-11-30 [PMID: 33266255] (Bioassay, Mouse) Bioassay Mouse
GN Yin, HR Jin, MJ Choi, A Limanjaya, K Ghatak, NN Minh, J Ock, MH Kwon, KM Song, HJ Park, HM Kim, YG Kwon, JK Ryu, JK Suh Pericyte-Derived Dickkopf2 Regenerates Damaged Penile Neurovasculature Through an Angiopoietin-1-Tie2 Pathway Diabetes, 2018-03-20;0(0):. 2018-03-20 [PMID: 29559443] (In Vivo, Mouse) In Vivo Mouse
B Gutbier, X Jiang, K Dietert, C Ehrler, J Lienau, P Van Slyke, H Kim, VC Hoang, JT Maynes, DJ Dumont, AD Gruber, N Weissmann, TJ Mitchell, N Suttorp, M Witzenrath Vasculotide reduces pulmonary hyperpermeability in experimental pneumococcal pneumonia Crit Care, 2017-11-13;21(1):274. 2017-11-13 [PMID: 29132435] (Bioassay) Bioassay
Yin , Guo Nan, Choi , Min Ji, Kim , Woo Jean, Kwon , Mi-Hye, Song , Kang-Moo, Park , Jin-Mi, Das , Nando Du, Kwon , Ki-Dong, Batbold , Dulguun, Oh , Goo Taeg, Koh , Gou Youn, Kim , Kyu-Won, Ryu , Ji-Kan, Suh , Jun-Kyu Inhibition of Ninjurin 1 restores erectile function through dual angiogenic and neurotrophic effects in the diabetic mouse. Proc Natl Acad Sci U S A, 2014-06-16;111(26):E2731-40. 2014-06-16 [PMID: 24979788] (In Vivo, Mouse) In Vivo Mouse
Popkov M, Jendreyko N, McGavern DB, Rader C, Barbas CF Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody. Cancer Res., 2005-02-01;65(3):972-81. 2005-02-01 [PMID: 15705898] (Binding Assay) Binding Assay

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Bioinformatics

Gene Symbol Tek
Uniprot