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Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 x 104 cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 80-90% will adhere after MnCl2 stimulation for 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse MAdCAM-1 protein
Mouse MAdCAM-1
(Gln22 - Thr365)
Accession # NP_038619		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln22 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Madcam1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
63 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
85-95 kDa, reducing conditions
Publications
Read Publications using
993-MC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF

  • hMAdCAM-1
  • MACAM1
  • MAdCAM1
  • MAdCAM-1
  • mucosal addressin cell adhesion molecule 1
  • mucosal addressin cell adhesion molecule-1
  • mucosal vascular addressin cell adhesion molecule 1

Background

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha 4 beta 7, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha 4 beta 7 binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta . MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha 4 beta 7-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha 4 beta 7 interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.

  1. Briskin, M.J. et al. (1993) Nature 363:461.
  2. Yang, X.D. et al. (1997) Diabetes 46:1542.
  3. Sampaio, S.O. et al. (1995) J. Immunol. 155:2477.
  4. Kraal, G. et al. (1995) Am. J. Pathol. 147:763.
  5. Berg, E.L. et al. (1993) Nature 366:695.
  6. Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.

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Publications for MAdCAM-1 (993-MC)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Adhesion Assay, Bioassay.


Filter By Application
Adhesion Assay
(1)
Bioassay
(10)
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Human
(1)
Mouse
(10)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 993-MC Applications Species
Sun, H;Lee, HS;Kim, SH;Fernandes de Lima, M;Gingras, AR;Du, Q;McLaughlin, W;Ablack, J;Lopez-Ramirez, MA;Lagarrigue, F;Fan, Z;Chang, JT;VanDyke, D;Spangler, JB;Ginsberg, MH; IL-2 can signal via chemokine receptors to promote regulatory T cells' suppressive function Cell reports 2023-08-29 [PMID: 37598341] (Bioassay, Mouse) Bioassay Mouse
Kamioka, Y;Ueda, Y;Kondo, N;Tokuhiro, K;Ikeda, Y;Bergmeier, W;Kinashi, T; Distinct bidirectional regulation of LFA1 and ?4?7 by Rap1 and integrin adaptors in T cells under shear flow Cell reports 2023-06-01 [PMID: 37267105] (Bioassay, Mouse) Bioassay Mouse
K Spinler, J Bajaj, T Ito, B Zimdahl, M Hamilton, A Ahmadi, CS Koechlein, N Lytle, HY Kwon, F Anower-E-K, H Sun, A Blevins, J Weeks, M Kritzik, J Karlseder, MH Ginsberg, PW Park, JD Esko, T Reya A stem cell reporter based platform to identify and target drug resistant stem cells in myeloid leukemia Nat Commun, 2020-11-26;11(1):5998. 2020-11-26 [PMID: 33243988] (Bioassay, Mouse) Bioassay Mouse
T Savinko, C Guenther, LM Uotila, M Llort Asen, S Yao, S Tojkander, SC Fagerholm Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking J. Immunol., 2018-03-26;0(0):. 2018-03-26 [PMID: 29581355] (Bioassay, Mouse) Bioassay Mouse
Kavanagh D, Suresh S, Newsome P, Frampton J, Kalia N Pretreatment of Mesenchymal Stem Cells Manipulates Their Vasculoprotective Potential While Not Altering Their Homing Within the Injured Gut. Stem Cells, 2015-06-29;33(9):2785-97. 2015-06-29 [PMID: 26124062] (Bioassay, Mouse) Bioassay Mouse
Halpert G, Eitan T, Voronov E, Apte R, Rath-Wolfson L, Albeck M, Kalechman Y, Sredni B Multifunctional activity of a small tellurium redox immunomodulator compound, AS101, on dextran sodium sulfate-induced murine colitis. J Biol Chem, 2014-04-24;289(24):17215-27. 2014-04-24 [PMID: 24764299] (Bioassay, Mouse) Bioassay Mouse
Wang X, Rodda L, Bannard O, Cyster J Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness. J Immunol, 2014-04-16;192(10):4601-9. 2014-04-16 [PMID: 24740506] (Bioassay, Mouse) Bioassay Mouse
Heidegger S, Kirchner S, Stephan N, Bohn B, Suhartha N, Hotz C, Anz D, Sandholzer N, Stecher B, Russmann H, Endres S, Bourquin C TLR activation excludes circulating naive CD8+ T cells from gut-associated lymphoid organs in mice. J Immunol, 2013-04-15;190(10):5313-20. 2013-04-15 [PMID: 23589622] (Adhesion Assay, Mouse) Adhesion Assay Mouse
Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH Difference in Th1 and Th17 lymphocyte adhesion to endothelium. J. Immunol., 2012-01-04;188(3):1421-30. 2012-01-04 [PMID: 22219321] (Bioassay, Mouse) Bioassay Mouse
Arthos J, Cicala C, Martinelli E, MacLeod K, Van Ryk D, Wei D, Xiao Z, Veenstra TD, Conrad TP, Lempicki RA, McLaughlin S, Pascuccio M, Gopaul R, McNally J, Cruz CC, Censoplano N, Chung E, Reitano KN, Kottilil S, Goode DJ, Fauci AS HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells. Nat. Immunol., 2008-02-10;9(3):301-9. 2008-02-10 [PMID: 18264102] (Bioassay, Human) Bioassay Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol Madcam1
Uniprot