Recombinant Mouse Complement Factor D Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Complement Factor D, also known as Adipsin, is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. It cleaves the C3b-bound Factor B, resulting in the formation of a C3bBcomplex, which functions as the alternative pathway C3 convertase (1-3). Factor D is primarily expressed in adipose tissue and is released into the circulation as a zymogen with a 5 amino acid (aa) N-terminal activation peptide (4-6). It is activated by MASP-1 mediated proteolytic removal of the activation peptide (6). Factor D triggered complement activation is important for the clearance of cell debris and control of inflammation following tissue damage (7). The C3 convertase generates C3a which binds to C3a R on pancreatic beta cells to induce insulin production and regulate glucose homeostasis (5). Mature mouse Complement Factor D shares 65% and 83% amino acid sequence identity with human and rat Complement Factor D, respectively. Its protease activity is regulated by reversible conformational changes, which is distinct from most serine proteases whose regulation involves either activation by zymogen processing or inactivation by binding to inhibitors. Compared to its cleavage of C3b-bound Factor B, Factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing Factor D activity (8).

Array 9207-SE

• Complement Factor D/Adipsin Antibodies
• Complement Factor D/Adipsin ELISA Kit
• Complement Factor D/Adipsin ELISAs
• Complement Factor D/Adipsin Proteins and Enzymes