Recombinant Mouse Complement Component C3a Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

C3a is an anaphylotoxin polypeptide comprising amino acids (aa) 671-748 of the Complement C3 precursor protein (1-4). Anaphylatoxins are proteolytically generated from the C3, C4 and C5 alpha chains by convertases formed by other complement fragments (2). They share 30-36% aa identity, and mediate inflammatory responses that vary in strength in the order C5a > C3a > C4a (2). Like C4a and C5a, the 78 aa, 9 kDa mouse C3a contains six conserved cysteine residues that form a knot structure and possess an overall basic charge (4, 5). It is not glycosylated (4). The C-terminal regions of C3a and C4a show antimicrobial activity, while C5a is chemotactic but not antimicrobial (5). Mouse C3a shares 94% aa sequence identity with rat, and 65-69% with human, guinea pig, bovine, porcine and canine C3a. C3a formation is common to all three pathways of complement activation: classical (antibody-mediated), lectin and alternative (1, 2). It binds the G-protein coupled C3a receptor (C3aR) on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells (6, 7). C3a contributes to both innate and adaptive immunity. It activates mast cells and neutrophils, triggering robust mast cell degranulation in airways during asthmatic allergen challenges (8). It enhances lipopolysaccharide-induced prostaglandin, cytokine and chemokine secretion by macrophages and other cells (1, 6, 9, 10). It assists in Th2-type inflammatory reactions, promotes Th1 cell maturation, and down-regulates regulatory T cell differentiation (8, 11). It stimulates leukocyte chemotaxis and smooth muscle contraction (8, 9).  Endogenous carboxypeptidase-N can remove the arginine at the C-terminus of the anaphylatoxins to create desArg forms (1). C3adesArg, also called ASP (Acylation-Stimulating Protein) is an adipocyte-derived protein that binds the C5L2 receptor (GPR77) and stimulates adipose tissue triglyceride synthesis (2, 7, 12). The anaphylactic activity of ASP is weaker than that of C3a (7, 10). C5L2 is also involved in C3a and C5a activity (12).

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), In Vivo.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using 8085-C3	Applications	Species
MB Kuhn, HS VandenBerg, AJ Reynolds, MD Carson, AJ Warner, AC LaRue, CM Novince, JD Hathaway-S C3a-C3aR signaling is a novel modulator of skeletal homeostasis Bone Reports, 2023-02-16;18(0):101662. 2023-02-16 [PMID: 36860797] (Bioassay, Mouse)	Bioassay	Mouse
A Cohen, EE Jeng, M Voorhies, J Symington, N Ali, RA Rodriguez, MC Bassik, A Sil Genome-scale CRISPR screening reveals that C3aR signaling is critical for rapid capture of fungi by macrophages PloS Pathogens, 2022-09-29;18(9):e1010237. 2022-09-29 [PMID: 36174103] (Bioassay, Mouse)	Bioassay	Mouse
S Ito, H Hashimoto, H Yamakawa, D Kusumoto, Y Akiba, T Nakamura, M Momoi, J Komuro, T Katsuki, M Kimura, Y Kishino, S Kashimura, A Kunitomi, M Lachmann, M Shimojima, G Yozu, C Motoda, T Seki, T Yamamoto, Y Shinya, T Hiraide, M Kataoka, T Kawakami, K Suzuki, K Ito, H Yada, M Abe, M Osaka, H Tsuru, M Yoshida, K Sakimura, Y Fukumoto, M Yuzaki, K Fukuda, S Yuasa The complement C3-complement factor D-C3a receptor signalling axis regulates cardiac remodelling in right ventricular failure Nature Communications, 2022-09-15;13(1):5409. 2022-09-15 [PMID: 36109509] (Bioassay, Rat)	Bioassay	Rat
M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells Journal of Neuroinflammation, 2022-07-14;19(1):182. 2022-07-14 [PMID: 35831910] (Bioassay, Mouse)	Bioassay	Mouse
B Gong, D Guo, C Zheng, Z Ma, J Zhang, Y Qu, X Li, G Li, L Zhang, Y Wang Complement C3a activates astrocytes to promote medulloblastoma progression through TNF-alpha Journal of Neuroinflammation, 2022-06-20;19(1):159. 2022-06-20 [PMID: 35725556] (Bioassay, Mouse)	Bioassay	Mouse
Z Zheng, YN Li, S Jia, M Zhu, L Cao, M Tao, J Jiang, S Zhan, Y Chen, PJ Gao, W Hu, Y Wang, C Shao, Y Shi Lung mesenchymal stromal cells influenced by Th2 cytokines mobilize neutrophils and facilitate metastasis by producing complement C3 Nature Communications, 2021-10-27;12(1):6202. 2021-10-27 [PMID: 34707103] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
FM Consonni, A Bleve, MG Totaro, M Storto, P Kunderfran, A Termanini, F Pasqualini, C Alì, C Pandolfo, F Sgambellur, G Grazia, M Santinami, A Maurichi, M Milione, M Erreni, A Doni, M Fabbri, L Gribaldo, E Rulli, MP Soares, V Torri, R Mortarini, A Anichini, A Sica Heme catabolism by tumor-associated macrophages controls metastasis formation Nature Immunology, 2021-04-26;22(5):595-606. 2021-04-26 [PMID: 33903766] (Bioassay, Mouse)	Bioassay	Mouse
BE Hsu, J Roy, J Mouhanna, RF Rayes, L Ramsay, S Tabariès, MG Annis, IR Watson, JD Spicer, S Costantino, PM Siegel C3a elicits unique migratory responses in immature low-density neutrophils Oncogene, 2020-02-04;0(0):. 2020-02-04 [PMID: 32020055] (Bioassay, Mouse)	Bioassay	Mouse
C Xiong, J Liu, D Lin, J Zhang, N Terrando, A Wu Complement activation contributes to perioperative neurocognitive disorders in mice J Neuroinflammation, 2018-09-04;15(1):254. 2018-09-04 [PMID: 30180861] (In Vivo, Mouse)	In Vivo	Mouse
CK Braun, M Kalbitz, R Halbgebaue, P Eisele, DAC Messerer, S Weckbach, A Schultze, S Braumüller, F Gebhard, MS Huber-Lang Early structural changes of the heart after experimental polytrauma and hemorrhagic shock PLoS ONE, 2017-10-30;12(10):e0187327. 2017-10-30 [PMID: 29084268] (ELISA (Standard), Mouse)	ELISA (Standard)	Mouse
HJ Bax, H Bowen, RL Beavil, R Chung, M Ward, AM Davies, TS Dodev, JM McDonnell, AJ Beavil, BJ Sutton, HJ Gould IgE Trimers Drive SPE-7 Cytokinergic Activity Sci Rep, 2017-08-15;7(1):8164. 2017-08-15 [PMID: 28811536] (Bioassay, Mouse)	Bioassay	Mouse
XJ Zhao, TM Larkin, MA Lauver, S Ahmad, AF Ducruet Tissue plasminogen activator mediates deleterious complement cascade activation in stroke PLoS ONE, 2017-07-10;12(7):e0180822. 2017-07-10 [PMID: 28700732] (Bioassay)	Bioassay
Alain Lamarre Complement Component 3 Regulates IFN-? Production by Plasmacytoid Dendritic Cells following TLR7 Activation by a Plant Virus-like Nanoparticle J. Immunol., 2016-11-18;0(0):. 2016-11-18 [PMID: 27864474] (In Vivo, Mouse)	In Vivo	Mouse
Show All 13 Publications. Collapse Publications.

Array 8085-C3

• Complement Component C3a Antibodies
• Complement Component C3a Proteins and Enzymes