Recombinant Mouse CD300f/LMIR3 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Leukocyte mono-immunoglobulin-like receptor 3 (LMIR3), also called CD300f and CLM-1, is a member of the paired immune receptor family within the immunoglobulin superfamily (1). Mature mouse LMIR3 consists of an extracellular domain (ECD) with one Ig-like V-type domain, a transmembrane segment, and a cytoplasmic domain that contains two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and immunoreceptor tyrosine-based switch motif (ITSM) (2). Alternate splicing generates additional isoforms with varying length C-terminal tails following the ECD (3). Within the ECD, mouse LMIR3 shares 43% amino acid (aa) sequence identity with human LMIR3. LMIR3 is expressed on the surface of dendritic cells, monocytes, granulocytes, and mast cells as well as on acute myeloid leukemia (AML) blasts (3, 4). Pervanadate treatment or antibody cross‑linking of LMIR3 induces phosphorylation of tyrosine residues in the cytoplasmic domain and the subsequent recruitment of phosphatases SHIP, SHP-1, SHP-2, and the p85 alpha subunit of PI3K (3, 5, 6). LMIR3 appears to exhibit a dual function in mast cells. LMIR3 functions as a negative regulator of MC activation through an inhibitory effect on Fc epsilon RI-mediated cytokine production in mast cells (5). Conversely, it enhances TLR4‑mediated signaling/cytokine production in mast cells through association with the activating signaling protein FcR gamma (5). Additionally, LMIR3 ligation can induce cell death and inhibit signaling through multiple receptors including Fc epsilon RI, LMIR4, SCF R, TLR2, TLR3, and TLR9 (3-8). In mouse, a splice variant of LMIR3 (known as DIgR2, with a 7 aa insertion in the ECD) inhibits CD4+ T cell activation and in vivo Th1 and CTL responses (9). LMIR3 is up‑regulated on monocytes surrounding experimentally-induced spinal cord demyelination and functions as a negative regulator of inflammation in the CNS (10).

Array 10967-LM

• CD300f/LMIR3/CD300LF Antibodies
• CD300f/LMIR3/CD300LF ELISA Kits
• CD300f/LMIR3/CD300LF Lysate
• CD300f/LMIR3/CD300LF Protein
• CD300f/LMIR3/CD300LF Proteins and Enzymes