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Recombinant Mouse CCL20/MIP-3 alpha Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
760-M3-025/CF
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Recombinant Mouse CCL20/MIP-3 alpha Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR6. The ED50 for the chemotactic effect is 2‑6 ng/mL.
Source
E. coli-derived mouse CCL20/MIP-3 alpha protein
Ala27-Met96
Accession #
N-terminal Sequence
Ala27
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl20
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
760-M3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL20/MIP-3 alpha Protein

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

MIP-3 alpha , also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20 - 28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71 and 63% amino acid sequence homology with rat and human MIP-3 alpha , respectively.

MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
  4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

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Recombinant Mouse
CCL20/MIP-3 alpha Pro ...
760-M3
Recombinant Mouse CCL20/MIP-3 alpha Protein
Species: Mu
Applications: Bioactivity

Publications for CCL20/MIP-3 alpha (760-M3)(14)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA Capture, In Vivo, Western Blot.


Filter By Application
Bioassay
(11)
Cell Culture
(1)
ELISA Capture
(1)
In Vivo
(2)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(14)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 760-M3 Applications Species
Z Maria, E Turner, A Agasing, G Kumar, RC Axtell Pertussis Toxin Inhibits Encephalitogenic T-Cell Infiltration and Promotes a B-Cell-Driven Disease during Th17-EAE International Journal of Molecular Sciences, 2021-03-13;22(6):. 2021-03-13 [PMID: 33805762] (Bioassay, Mouse) Bioassay Mouse
K Luo, JT Gordy, F Zavala, RB Markham A chemokine-fusion vaccine targeting immature dendritic cells elicits elevated antibody responses to malaria sporozoites in infant macaques Scientific Reports, 2021-01-13;11(1):1220. 2021-01-13 [PMID: 33441615] (ELISA Capture, Mouse) ELISA Capture Mouse
CG Da Silva, MGM Camps, TMWY Li, L Zerrillo, CW Löwik, F Ossendorp, LJ Cruz Effective chemoimmunotherapy by co-delivery of doxorubicin and immune adjuvants in biodegradable nanoparticles Theranostics, 2019-08-21;9(22):6485-6500. 2019-08-21 [PMID: 31588231] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
AS Wilson, HD Law, CB Knobbe-Tho, CJ Kearney, J Oliaro, C Binsfeld, G Burgio, L Starrs, D Brenner, KL Randall, A Brüstle Protection from EAE in DOCK8 mutant mice occurs despite increased Th17 cell frequencies in the periphery Eur. J. Immunol., 2019-02-20;0(0):. 2019-02-20 [PMID: 30729501] (Bioassay, Mouse) Bioassay Mouse
G Porras, MS Ayuso, C González-M Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice Int. J. Biochem. Cell Biol., 2018-03-28;99(0):72-79. 2018-03-28 [PMID: 29604348] (Cell Culture, Mouse) Cell Culture Mouse
Valentina Cecchinato Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization J. Immunol, 2016-11-28;0(0):. 2016-11-28 [PMID: 27895171] (Bioassay, Mouse) Bioassay Mouse
SW Huang, C Walker, J Pennock, K Else, W Muller, MJ Daniels, C Pellegrini, D Brough, G Lopez-Cast, SM Cruickshan P2X7 receptor-dependent tuning of gut epithelial responses to infection Immunol. Cell Biol, 2016-08-25;95(2):178-188. 2016-08-25 [PMID: 27559003] (Bioassay, Mouse) Bioassay Mouse
SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin J. Immunol, 2016-02-05;196(6):2514-25. 2016-02-05 [PMID: 26851219] (Bioassay, Mouse) Bioassay Mouse
Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK. PLoS ONE, 2015-04-24;10(4):e0125340. 2015-04-24 [PMID: 25909640] (Bioassay, Mouse) Bioassay Mouse
Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis. PLoS ONE, 2014-04-03;9(4):e93057. 2014-04-03 [PMID: 24699535] (In Vivo, Mouse) In Vivo Mouse
Show All 14 Publications.

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Bioinformatics

Gene Symbol Ccl20
Uniprot