Reactivity | MuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Details of Functionality | Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is 2-10 ng/mL. |
Source | E. coli-derived mouse CCL2/JE/MCP-1 protein Gln24-Arg96 |
Accession # | |
N-terminal Sequence | Gln24 |
Protein/Peptide Type | Recombinant Proteins |
Gene | Ccl2 |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note | <0.01 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 8.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile PBS. |
CCL2, also called monocyte chemotactic protein-1 (MCP-1) or JE, is a member of the C-C or beta chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Mouse CCL2 cDNA encodes a 148 amino acid (aa) precursor protein with a 23 aa signal peptide and a 125 aa mature protein (1). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, occurs naturally by metalloproteinase cleavage and downregulates activity but not receptor binding (3). Mouse CCL2 forms a broad band around 25 kDa on SDS-PAGE due to non-covalent dimerization and variable carbohydrate content (1). Mouse and rat express a form of CCL2 that is extended by 49 aa compared to other species. Mature mouse CCL2 shares 82% amino acid (aa) identity with rat CCL2 over the entire sequence, and 58%, 56%, 55%, 53% and 53% aa identity with human, equine, porcine, bovine and canine CCL2, respectively, over aa 24 ‑ 101. Human CCL2 can, however, induce a limited response in rodent cells, and mouse CCL2 has full activity on human cells (2, 4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2). In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4+ T cells (5). CCL2-mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7). When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay.
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