Home » Cadherin-6/KCAD » Cadherin-6/KCAD Proteins » Recombinant Mouse Cadherin-6/KCAD Protein, CF

Recombinant Mouse Cadherin-6/KCAD Protein, CF

 (1 Publication)
 See All Related
Submit a Review

Images

 
There are currently no images for Cadherin-6/KCAD (6826-CA).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals offers 100% Guarantee

Recombinant Mouse Cadherin-6/KCAD Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 0.2-1.0 μg/mL after a 30 minutes incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse Cadherin-6/KCAD protein
Met1-Ala615 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asn22 (pro-protein) & Ser54 (mature protein)
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
67.0 kDa (pro-protein) & 63.3 kDa (mature protein).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
90-105 kDa, reducing conditions
Publications
Read Publication using
6826-CA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in DPBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Cadherin-6/KCAD Protein, CF

  • cadherin 6, type 2, K-cadherin (fetal kidney)
  • cadherin, fetal kidney
  • Cadherin6
  • Cadherin-6
  • CDH6
  • KCAD
  • K-cadherin (fetal kidney)
  • K-Cadherin

Background

Cadherin‑6, also known as KCAD or K‑Cadherin, is a 110‑120 kDa type I transmembrane glycoprotein belonging to the classical Cadherin superfamily of
calcium‑dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Mouse Cadherin‑6 is synthesized with a 18 amino acid (aa) signal peptide and a 35 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 562 amino acid (aa) extracellular domain (ECD) that contains five tandem Cadherin repeats, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (3). Within the ECD, mouse Cadherin‑6 shares 96% aa sequence identity with human and rat Cadherin‑10. It interacts homotypically as well as heterotypically with Cadherin‑9 and more weakly with Cadherins‑7, ‑10, and ‑14 (4, 5). Cadherin‑6 is expressed primarily in the kidney and brain, both during development and in the adult (6‑9). It is enriched in renal proximal tubule epithelial cells and their precursors at sites of cell‑cell contact as well as on basolateral membranes (7, 10, 11). In the nervous system, Cadherin‑6 is neuronally expressed in discrete regions of the neural plate, neural crest cells that give rise to peripheral nerves, and the brain (3, 6). Cadherin‑6 is additionally expressed in the developing retina, osteoclasts and their progenitors, ovarian epithelium, and endometrium during the follicular phase of the menstrual cycle (12‑15). It is also expressed in carcinomas of the kidney, ovary, and liver (8, 10, 11, 14).
  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  3. Inoue, T. et al. (1997) Dev. Biol. 183:183.
  4. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  5. Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.
  6. Inoue, T. et al. (1998) Dev. Dyn. 211:338.
  7. Xiang Y.-Y. et al. (1994) Cancer Res. 54:3034.
  8. Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
  9. Cho, E.A. et al. (1998) Development 125:803.
  10. Paul, R. et al. (1997) Cancer Res. 57:2741.
  11. Shimazui, T. et al. (2000) Eur. Urol. 38:331.
  12. Ruan, G. et al. (2006) Mech. Dev. 123:881.
  13. Mbalaviele, G.  et al. (1998) J. Cell Biol.  141:1467.
  14. Sellar, G.C. et al. (2001) Cancer Res.  61:6977.
  15. Getsios, S. et al. (1998) Dev. Dyn. 211:238.

Customers Who Viewed This Item Also Viewed...

Cadherin-11
Antibody (283416)
MAB1790
Immunohistochemistry Cadherin-11 Antibody (283416)
Simple Western Cadherin-11 Antibody (283416)
Western Blot Cadherin-11 Antibody (283416)
(1 Review)
(11 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
E-Cadherin
Antibody [Unconjugate
AF748
Immunocytochemistry E-Cadherin Antibody [Unconjugated]
Immunohistochemistry E-Cadherin Antibody [Unconjugated]
Immunocytochemistry E-Cadherin Antibody [Unconjugated]
(143 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, ICC, IHC, Simple Western, WB
Cadherin-8
[Unconjugated]
188-C8
Cadherin-8 [Unconjugated]
Species: Hu
Applications: BA
N-Cadherin
Antibody (13A9)
NBP1-48309
Flow (Intracellular) N-Cadherin Antibody (13A9)
Flow Cytometry N-Cadherin Antibody (13A9)
Immunocytochemistry/ Immunofluorescence N-Cadherin Antibody (13A9)
(2 Reviews)
(42 Publications)
Species: Hu, Mu, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Catenin alpha 1
Antibody
NBP2-15723
Western Blot Catenin alpha 1 Antibody
Western Blot Catenin alpha 1 Antibody
Immunoprecipitation Catenin alpha 1 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB

Cadherin-4/R-Cadherin
AF6677
Western Blot Cadherin-4/R-Cadherin Antibody
Immunohistochemistry Cadherin-4/R-Cadherin Antibody
(1 Publication)
Species: Mu
Applications: IHC, WB
Cadherin-18
Antibody (6F7)
H00001016-M01
Immunohistochemistry-Paraffin Cadherin-18 Antibody (6F7)
Western Blot Cadherin-18 Antibody (6F7)
Sandwich ELISA Cadherin-18 Antibody (6F7)
(1 Publication)
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
Cadherin-10
6770-CA
Cadherin-10
Species: Hu
Applications: BA
Cadherin-20
5604-CA
Cadherin-20
(1 Publication)
Species: Hu
Applications: BA
SS18L1 Antibody
NBP2-20486
Immunoprecipitation SS18L1 Antibody
Immunohistochemistry-Paraffin SS18L1 Antibody
Immunocytochemistry/ Immunofluorescence SS18L1 Antibody
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Protocadherin-10
[Unconjugated]
6510-CA
Protocadherin-10 [Unconjugated]
Species: Hu
Applications: BA

M-Cadherin/Cadherin-15 ...
AF4096
Immunocytochemistry M-Cadherin/Cadherin-15 Antibody [Unconjugated]
Flow Cytometry M-Cadherin/Cadherin-15 Antibody [Unconjugated]
Western Blot M-Cadherin/Cadherin-15 Antibody [Unconjugated]
(3 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, WB
Cadherin-7
6774-CA
Cadherin-7
(1 Publication)
Species: Hu
Applications: BA
Protocadherin-17
Antibody
NBP1-80943
Immunocytochemistry/ Immunofluorescence Protocadherin-17 Antibody
Immunohistochemistry-Paraffin Protocadherin-17 Antibody
Immunohistochemistry-Paraffin Protocadherin-17 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Cadherin-19
Antibody
H00028513-B01P
Western Blot Cadherin-19 Antibody
Western Blot Cadherin-19 Antibody
(1 Publication)
Species: Hu
Applications: WB
BMP-4
[Unconjugated]
314-BP
SDS-PAGE BMP-4 [Unconjugated]
Bioactivity BMP-4 [Unconjugated]
(531 Publications)
Species: Hu
Applications: BA, BA
Carbonic Anhydrase
IX/CA9 Antibody - BSA ...
NB100-417
Flow Cytometry Carbonic Anhydrase IX/CA9 Antibody - BSA Free
Immunoblotting Carbonic Anhydrase IX/CA9 Antibody - BSA Free
Immunoblotting Carbonic Anhydrase IX/CA9 Antibody - BSA Free
(7 Reviews)
(292 Publications)
Species: Hu, Mu, Pl, Rt
Applications: ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PLA, Simple Western, WB
Recombinant Mouse
Cadherin-6/KCAD Prote ...
6826-CA
Recombinant Mouse Cadherin-6/KCAD Protein, CF
Species: Mu
Applications: Bioactivity

Publications for Cadherin-6/KCAD (6826-CA)(1)

We have publications tested in 1 confirmed species: N/A.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(1)
All Applications
Filter By Species
N/A
(1)
All Species

Reviews for Cadherin-6/KCAD (6826-CA) (0)

There are no reviews for Cadherin-6/KCAD (6826-CA). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Cadherin-6/KCAD (6826-CA) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Cadherin-6/KCAD Products

Blogs on Cadherin-6/KCAD

There are no specific blogs for Cadherin-6/KCAD, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Cadherin-6/KCAD Protein, CF and receive a gift card or discount.


Bioinformatics

Uniprot