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Recombinant Human VCAM-1/CD106 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
809-VR-200
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Recombinant Human VCAM-1/CD106 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human VCAM-1/CD106 protein
Phe25-Glu698, with a substitution of Arg322Gly and an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
VCAM1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
90-95 kDa, reducing conditions
Publications
Read Publications using
809-VR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VCAM-1/CD106 Protein, CF

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

Human VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta , IL-4, TNF-alpha and IFN‑ gamma . VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7.

During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.

ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

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Recombinant Human
VCAM-1/CD106 Protein, ...
809-VR
Recombinant Human VCAM-1/CD106 Protein, CF
Species: Hu
Applications: Bioactivity

Publications for VCAM-1/CD106 (809-VR)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, ELISA (Standard).


Filter By Application
Bioassay
(9)
ELISA (Standard)
(1)
All Applications
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Human
(8)
All Species
Showing Publications 1 - 10 of 10.
Publications using 809-VR Applications Species
Harich, OO;Gavriliuc, OI;Ordodi, VL;Tirziu, A;Paunescu, V;Panaitescu, C;Bojin, MF; In Vitro Study of the Multimodal Effect of Na+/K+ ATPase Blocker Ouabain on the Tumor Microenvironment and Malignant Cells Biomedicines 2023-08-05 [PMID: 37626702] (Bioassay, Human) Bioassay Human
K Sottoriva, NY Paik, Z White, T Bandara, L Shao, T Sano, K Pajcini Notch-IL21 signaling axis primes bone marrow T cell progenitor expansion JCI Insight, 2022-05-09;0(0):. 2022-05-09 [PMID: 35349492] (Bioassay, Human) Bioassay Human
C Silvestre-, Q Braster, K Wichapong, EY Lee, JM Teulon, N Berrebeh, J Winter, JM Adrover, GS Santos, A Froese, P Lemnitzer, A Ortega-Góm, R Chevre, J Marschner, A Schumski, C Winter, L Perez-Oliv, C Pan, N Paulin, T Schoufour, H Hartwig, S González-R, F Kamp, RTA Megens, KA Mowen, M Gunzer, L Maegdefess, T Hackeng, E Lutgens, M Daemen, J von Blume, HJ Anders, VO Nikolaev, JL Pellequer, C Weber, A Hidalgo, GAF Nicolaes, GCL Wong, O Soehnlein Externalized histone H4 orchestrates chronic inflammation by inducing lytic cell death Nature, 2019-05-01;569(7755):236-240. 2019-05-01 [PMID: 31043745] (Bioassay, Human) Bioassay Human
MJ Aldape, A Tao, DD Heeney, ER McIndoo, JM French, D Xu Experimental identification and computational characterization of a novel extracellular metalloproteinase produced by Clostridium sordellii RSC Adv, 2017-03-02;7(23):13928-13938. 2017-03-02 [PMID: 28515901] (Bioassay) Bioassay
Chakraborty S, Hu S, Wu S, Karmenyan A, Chiou A The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET. PLoS ONE, 2015-03-20;10(3):e0121399. 2015-03-20 [PMID: 25793408] (Bioassay, Human) Bioassay Human
Lee J, Sohn J, Ryu S, Hong C, Moon K, Park J A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling. J Cell Mol Med, 2013-07-16;17(10):1271-81. 2013-07-16 [PMID: 23855490] (Bioassay, Human) Bioassay Human
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012-02-17;378(1):72-80. 2012-02-17 [PMID: 22366959] (ELISA (Standard)) ELISA (Standard)
Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients. Leukemia, 2011-12-02;26(5):1098-105. 2011-12-02 [PMID: 22134715] (Bioassay, Human) Bioassay Human
Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. J. Hepatol., 2009-07-30;51(4):734-49. 2009-07-30 [PMID: 19703720] (Bioassay, Human) Bioassay Human
Arthos J, Cicala C, Martinelli E, MacLeod K, Van Ryk D, Wei D, Xiao Z, Veenstra TD, Conrad TP, Lempicki RA, McLaughlin S, Pascuccio M, Gopaul R, McNally J, Cruz CC, Censoplano N, Chung E, Reitano KN, Kottilil S, Goode DJ, Fauci AS HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells. Nat. Immunol., 2008-02-10;9(3):301-9. 2008-02-10 [PMID: 18264102] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol VCAM1
Uniprot