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Recombinant Human TGF-beta 3 (CHO-expressed) Protein

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Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.01-0.04 ng/mL.
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.01-0.04 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
8420-B3-005/CF
Carrier Free
8420-B3-025/CF
Carrier Free
8420-B3-100/CF
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Recombinant Human TGF-beta 3 (CHO-expressed) Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.01-0.04 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 3 protein
Ala301-Ser412
Accession #
N-terminal Sequence
Ala301
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB3
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
9-11 kDa, reducing conditions
Publications
Read Publications using
8420-B3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 3 (CHO-expressed) Protein

  • ARVD
  • ARVD1
  • FLJ16571
  • LDS5
  • RNHF
  • TGFB3
  • TGFbeta 3
  • TGF-beta 3
  • TGF-beta3
  • TGF-beta-3
  • transforming growth factor beta-3
  • transforming growth factor, beta 3

Background

TGF­-beta 3 (transforming growth factor-beta 3) is a member of a TGF­-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and ­ beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial-­mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF-­ beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF­-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin-­like convertase to generate a 220 aa latency-­associated peptide (LAP) and a 112 aa mature TGF­-beta 3 (10, 11). Mature human TGF-­ beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF-­ beta 3, respectively. TGF-beta 3 is secreted as a latent complex. This latent form of TGF-beta 3 is activated by integrins, thrombospondin-1, plasmin, and matrix metalloproteases (12, 13). It can also be activated by extreme pH and reactive oxygen species (1-5, 12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-­ beta RI or ALK-­1, to modulate transcription through Smad phosphorylation (14-16). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF-­ beta RIII and endoglin) and the subsequent initiation of Smad­-dependent or -independent signaling pathways (15, 17, 18).

  1. Barrio, M.C. et al. (2014) Cells Tissues Organs. [Epub ahead of print; PMID 24861080].
  2. Doetschman, T. et al. (2012) Genesis 50:59.
  3. Mittl, P.R. et al. (1996) Protein Sci. 5:1261.
  4. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  5. Wahl, S.M. et al. (2006) Immunol. Rev. 213:213.
  6. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  7. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  8. Jin, J.Z. et al. (2014) Dev. Dyn. [Epub ahead of print; PMID 25104574].
  9. Tang, Q.O. et al. (2009) Expert Opin. Biol Ther. 9:689.
  10. Derynck, R. et al. (1988) EMBO J. 7:3737.
  11. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  12. Munger, J.S. et al. (1997) Kidney Int 51:1376.
  13. Wipff, P.J. and B. Hinz (2008) Eur J Cell Biol 87:601.
  14. Cui, X.M. and C.F. Shuler (2000) Int. J. Dev. Biol. 44:397.
  15. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  16. Nakajima, A. et al. (2007) Dev. Dyn. 236:791.
  17. Iwata, J. et al. (2012) J. Clin. Invest. 122:873.
  18. Gatza, C.E. et al. (2010) Cell. Signal. 22:1163.

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Publications for TGF-beta 3 (8420-B3)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Equine.

We have publications tested in 2 applications: Bioassay, Cell Culture.


Filter By Application
Bioassay
(12)
Cell Culture
(1)
All Applications
Filter By Species
Human
(9)
Mouse
(2)
Equine
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 8420-B3 Applications Species
De Martin, A;Stanossek, Y;L�tge, M;Cadosch, N;Onder, L;Cheng, HW;Brandstadter, JD;Maillard, I;Stoeckli, SJ;Pikor, NB;Ludewig, B; PI16+ reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches Nature immunology 2023-05-18 [PMID: 37202490] (Bioassay, Human) Bioassay Human
Ideno H, Imaizumi K, Shimada H, Sanosaka T, Nemoto A, Kohyama J, Okano H Human PSCs determine the competency of cerebral organoid differentiation via FGF signaling and epigenetic mechanisms. iScience, 2022-09-16;25(10):105140. 2022-09-16 [PMID: 36185382] (Bioassay, Human) Bioassay Human
Watanabe M, Buth J, Haney J, Vishlaghi N, Turcios F, Elahi L, Gu W, Pearson C, Kurdian A, Baliaouri N, Collier A, Miranda O, Dunn N, Chen D, Sabri S, Torre-Ubieta L, Clark A, Plath K, Christofk H, Kornblum H, Gandal M, Novitch B TGFbeta superfamily signaling regulates the state of human stem cell pluripotency and capacity to create well-structured telencephalic organoids. Stem Cell Reports, 2022-09-29;17(10):2220-2238. 2022-09-29 [PMID: 36179695] (Bioassay, Human) Bioassay Human
K Takahashi, KA Podyma-Ino, M Saito, S Sakakitani, A Sugauchi, K Iida, S Iwabuchi, D Koinuma, K Kurioka, T Konishi, S Tanaka, A Kaida, M Miura, S Hashimoto, M Okada, T Uchihashi, K Miyazono, T Watabe TGF-beta generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3 Cell Reports, 2022-09-27;40(13):111411. 2022-09-27 [PMID: 36170816] (Bioassay, Human) Bioassay Human
AM Earley, LF Burbulla, D Krainc, R Awatramani Identification of ASCL1 as a determinant for human iPSC-derived dopaminergic neurons Scientific Reports, 2021-11-15;11(1):22257. 2021-11-15 [PMID: 34782629] (Bioassay, Human) Bioassay Human
de Rus Jacquet A, Tancredi J, Lemire A, DeSantis M, Li W, O'Shea E The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson&#039;s disease. Elife, 2021-09-30;10(0):. 2021-09-30 [PMID: 34590578] (Bioassay, Human) Bioassay Human
S Garnica-Ga, SH Korntner, I Skoufos, A Tzora, N Diakakis, N Prassinos, DI Zeugolis Hyaluronic Acid as Macromolecular Crowder in Equine Adipose-Derived Stem Cell Cultures Cells, 2021-04-09;10(4):. 2021-04-09 [PMID: 33918830] (Bioassay, Human) Bioassay Human
de Rus Jacquet A Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes. Curr Protoc Cell Biol, 2019-12-01;85(1):e98. 2019-12-01 [PMID: 31763766] (Bioassay, Human) Bioassay Human
SP Roth, W Brehm, C Gro beta , P Scheibe, S Schubert, J Burk Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering Int J Mol Sci, 2019-11-03;20(21):. 2019-11-03 [PMID: 31684150] (Cell Culture, Equine) Cell Culture Equine
J Ren, M Smid, J Iaria, DCF Salvatori, H van Dam, HJ Zhu, JWM Martens, P Ten Dijke Cancer-associated fibroblast-derived Gremlin 1 promotes breast cancer progression Breast Cancer Res., 2019-09-18;21(1):109. 2019-09-18 [PMID: 31533776] (Bioassay, Human) Bioassay Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol TGFB3
Uniprot