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Recombinant Human NF-L Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Format
Carrier-Free

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Recombinant Human NF-L Protein, CF Summary

Details of Functionality
Bioassay data are not available.
Source
E. coli-derived human NF-L protein
Ser2-Asp338, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser2
Protein/Peptide Type
Innovator Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity not tested
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
41 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 500 μg/mL in 4 mM HCl..

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NF-L Protein, CF

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neurofilament light polypeptide (NF-L), also known as 68 kDa neurofilament protein, and neurofilament triplet L protein, is an approximately 68 kDa member of the neuronal intermediate filament family that plays a role in the maintenance of neuronal caliber and possibly the pathogenesis of motor neuron diseases (1, 2). Out of the three intermediate filament proteins (NF-L, NF-M, NF-H), NF-L is the most abundant. NF-L is a 542 amino acid (aa) cytoskeletal protein which consists of a 91 aa N-terminal head, a 304 aa alpha -helical rod, and a 147 aa C-terminal tail (3). Within the extracellular domains, human NF-L shares 95% and 96% sequence identity with mouse and rat NF-L, respectively.
  1. Monteiro, MJ. et al. (1990) J. Cell. Biol. 111:1543.
  2. Xu, Z. et al. (1993) Cell. 73:23.
  3. Adebola, A. et al. (2015) Hum. Mol. Genet. 24:2163.

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Recombinant Human
NF-L Protein, CF
9715-NF
Recombinant Human NF-L Protein, CF
Species: Hu

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