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Recombinant Human Mer Fc Chimera Protein, CF

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 (1 Review)
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Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Human Mer Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human (rh) Mer Fc Chimera at 10 µg/mL (100 µL/well) can bind rhGas6 (Catalog # 885-GSB) with a linear range of 0.03-1 µg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Mer protein
Human Mer
(Arg26-Ala499)
Accession # AAB60430.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg26
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
MERTK
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
78 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-110 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 5
using
891-MR in the following application:
Publications
Read Publications using
891-MR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Mer Fc Chimera Protein, CF

  • c-Eyk
  • c-mer proto-oncogene tyrosine kinase
  • C-mer
  • EC 2.7.10
  • EC 2.7.10.1
  • MER receptor tyrosine kinase
  • Mer
  • MerTK
  • MGC133349
  • Receptor tyrosine kinase MerTK
  • RP38Proto-oncogene c-Mer
  • STK kinase
  • tyrosine-protein kinase Mer

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

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Publications for Mer (891-MR)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, Immunoprecipitation, SPR.


Filter By Application
Bioassay
(3)
Immunoprecipitation
(1)
SPR
(1)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using 891-MR Applications Species
F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages Cell Death & Disease, 2021-05-25;12(6):538. 2021-05-25 [PMID: 34035216] (SPR, Human) SPR Human
Y Zhao, X Wu, X Li, LL Jiang, X Gui, Y Liu, Y Sun, B Zhu, JC Piña-Cresp, M Zhang, N Zhang, X Chen, G Bu, Z An, TY Huang, H Xu TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function Neuron, 2018-03-07;97(5):1023-1031.e7. 2018-03-07 [PMID: 29518356] (Immunoprecipitation, Human) Immunoprecipitation Human
Zizzo G, Hilliard B, Monestier M, Cohen P Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol, 2012-08-31;189(7):3508-20. 2012-08-31 [PMID: 22942426] (Bioassay, Human) Bioassay Human
Cerchia , Laura, Esposito , Carla L, Camorani , Simona, Rienzo , Anna, Stasio , Loredana, Insabato , Luigi, Affuso , Andrea, de Franciscis , Vittorio Targeting Axl with an high-affinity inhibitory aptamer. Mol Ther, 2012-08-21;20(12):2291-303. 2012-08-21 [PMID: 22910292] (Bioassay, Human) Bioassay Human
Uehara H, Shacter E Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells. J. Immunol., 2008-02-15;180(4):2522-30. 2008-02-15 [PMID: 18250462] (Bioassay, Human) Bioassay Human

Review for Mer (891-MR) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 891-MR:
Filter by Applications
Immunoassay Standard
(1)
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Images Ratings Applications Species Date Details
Immunoassay Standard Mer 891-MR
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reviewed by:
Verified Customer
 Immunoassay Standard 03/23/2018
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Summary

ApplicationImmunoassay Standard
LotCXK0416051

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Immunoassay Standard Mer 891-MR
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Bioinformatics

Gene Symbol MERTK
Uniprot