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Recombinant Human IL-15 GMP Protein, CF

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GMP-grade Recombinant Human IL-15 (Catalog # BT-015-GMP) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50for this effect is 0.300-2.60 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP IL-15.
GMP-grade Recombinant Human IL-15 (Catalog # BT-015-GMP) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50for this effect is 0.300-2.60 ng/mL. Three independent lots were tested ...read more
2 μg/lane of GMP-grade Recombinant Human IL-15 (Catalog # BT-015-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a single band at 9 kDa.
Two samples of GMP-grade human IL-15 (Catalog number BT-015-GMP, green circles) were interpolated with the human IL-15 Quantikine ELISA (D1500) standard curve (black circles) using 4PL regression analysis. The human ...read more
Thirteen samples of GMP-grade human IL-15 (Catalog number BT-015-GMP, green circles) were interpolated with the Simple Plex Human IL-15 cartridge (SPCKB-PS-000500) standard curve (black circles) using 4PL logistic ...read more
Three independent lots of Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) were analyzed by Maurice icIEF using native fluorescence detection (Mkr 5.85 and 9.99 are pI Markers). Profiles from the three runs ...read more
Three independent lots of Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) were analyzed by Maurice CE-SDS PLUS (IS is an Internal Standard). A gel-like representation of the purity analysis data (inset) can ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
BT-015-GMP-025/LQ
Carrier Free
BT-015-GMP-010
Carrier Free
BT-015-GMP-01M/LQ
Carrier Free
BT-015-GMP-01M
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Recombinant Human IL-15 GMP Protein, CF Summary

Additional Information
Animal-Free
Details of Functionality
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.300-2.60 ng/mL. The specific activity of recombinant human IL-15 is >2.00 x 108 units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554).
Source
E. coli-derived human IL-15 protein
Asn49-Ser162
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.
Accession #
N-terminal Sequence
Asn49-Trp-Val-Asn-Val-Ile-Ser-Asp-Leu-Lys
Protein/Peptide Type
GMP Recombinant Proteins
Purity
>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining. The molecular weight by mass spectrometry is 12761 Da ± 5 Da
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
9 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining. The molecular weight by mass spectrometry is 12761 Da ± 5 Da
Reconstitution Instructions
Reconstitute at 100-500 μg/mL in PBS.

Notes

END USER TERMS OF USE OF PRODUCT

The following terms are offered to you upon your acceptance of these End User Terms of Use of Product. By using this product, you indicate your acknowledgment and agreement to these End User Terms of Use of Product. If you do not agree to be bound by and comply with all of the provisions of these End User Terms of Use of Product, you should contact your supplier of the product and make arrangements to return the product.

We suggest you print and retain a copy of these End User Terms of Use of Product for your records.

The End User is aware that R&D Systems, Inc. sells GMP products for preclinical or clinical ex vivo use and not for in vivo use. The End User further agrees, as a condition of the sale of R&D Systems' GMP products that: a) the End User will not use this GMP Product in any procedure wherein the product may be directly or indirectly administered to humans, unless the End User has obtained, or prior to their use will have obtained, an Investigational New Drug (IND) exemption from the FDA and will use the product only in accordance with the protocols of such IND and of the Institutional Review Board overseeing the proposed research, or b) the End User will use the products outside of the United States in accordance with the protocols of research approved by the Institutional Review Board or authorized ethics committee and regulatory agencies to which the End User is subject to in their territory.

R&D Systems, Inc. has the right, at its sole discretion, to modify, add or remove any terms or conditions of these End User Terms of Use without notice or liability to you. Any changes to these End User Terms of Use are effective immediately following the printing of such changes on this product insert. The most recent version of these End User Terms of Use of Product may be found at: RnDSystems.com/Legal.

You agree to review these End User Terms of Use of Product to ensure any subsequent use by you of R&D Systems' GMP Products following changes to these End User Terms of Use of Product constitutes your acceptance of all such changes.

 

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This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-15 GMP Protein, CF

  • IL15
  • IL-15
  • IL-15MGC9721
  • interleukin 15
  • interleukin-15

Background

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). IL-15 binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). 

The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (12 - 16). An important component of these functions is the ability of IL‑15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18). 

Immunotherapy treatment with recombinant IL-15 has the advantage of not stimulating Treg cells like IL-2 does but has the drawback of associated toxicity at higher doses. This has led to increased investigation on mitigating IL-15 toxicity and combination immunotherapy approaches using immune checkpoint inhibitors (19, 20). Preclinical and early clinical studies have shown the potential of also using IL-15 in combination with cancer vaccines to improve their anti-tumor response (20). 

IL-15 can also be used for the preconditioning of CAR T cells or for engineering cells to express IL-15 in vivo. Adoptive cell transfer of NK cells engineered to express CD19 and IL-15 were well tolerated in patients with CD19-positive cancers (20). IL-15 can be used in combination with other cytokines like IL-21 to increase the efficiency of NK cell expansion and maturation in stem cell culture protocols (21). The combination of IL-15 with IL-7 also promotes expansion of early-differentiated CD8+ T cells in culture with the added benefit of decreasing Treg cell generation, unlike IL-2, for adoptive cell transfer in cancer immunotherapy (22). GMP IL-7 and GMP IL-15 are commonly used in combination for ex vivo expansion of T cells for cellular therapies.
  1. De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  2. Grabstein, K. et al. (1994) Science 264:965.
  3. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  4. Giri, J.G. et al. (1995) EMBO J. 14:3654.
  5. Giri, J. et al. (1994) EMBO J. 13:2822.
  6. Dubois, S. et al. (2002) Immunity 17:537.
  7. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
  8. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  9. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  10. Bergamaschi, C. et al. (2012) Blood 120:e1.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  12. Mortier, E. et al. (2003) J. Exp. Med. 205:1213.
  13. Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
  14. Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
  15. Xia, J. et al. (2010) Clin. Immunol. 134:130.
  16. Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
  17. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
  18. Barra, N.G. et al. (2010) Obesity 18:1601.
  19. Xue, D. et al. (2021) Antib Ther. 4:123.
  20. Wolfarth, A.A. et al. (2022) Immune Netw. 22:e5.
  21. Oberoi, P. et al. (2020) Cells. 9:811.
  22. Chamucero-Millares, J.A. et al. (2021) Cellular Immunol. 360:104257.

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Recombinant Human
IL-15 GMP Protein, CF
BT-015-GMP
Recombinant Human IL-15 GMP Protein, CF
Species: Hu
Applications: Bioactivity

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