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Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF

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When Human CX3CL1/Fractalkine MAb (Catalog # MAB3651) is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag binds with an ED50 of 0.15‑0.9 μg/mL.
When Human CX3CL1/Fractalkine MAb (Catalog # MAB3651) is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag binds with an ED50 of 0.15‑0.9 μg/mL.
2 μg/lane of Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag Protein (Catalog # AVI365) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity, Bioactivity
Format
Carrier-Free

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Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF Summary

Additional Information
Biotinylated
Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED50 for this effect is 20-100 ng/mL. Measured by its binding ability in a functional ELISA.
When Recombinant Human CX3CL1/Fractalkine monoclonal antibody (Catalog # MAB3651) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag binds with ED50 of 0.15-0.9 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human CX3CL1/Fractalkine protein
Human CX3CL1/Fractalkine
(Gln25-Thr338)
Accession # P78423.1		 HHHHHH  Avi-tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Gln25, inferred from enzymatic pyroglutamate treatment revealing His26
Structure / Form
Biotinylated via Avi-tag
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
  • Bioactivity2
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
88-103 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

Fractalkine, also known as CX3C motif chemokine 1, CX3CL1, neurotactin and small-inducible cytokine D1, is the only member of the CX3C subfamily of the chemokine superfamily (1). Mature human Fractalkine consists of an N-terminal chemokine domain with a CX3C motif and a mucin-like stalk region in the extracellular domain (ECD), a transmembrane segment and a short cytoplasmic domain (1, 2). The soluble form of Fractalkine is generated via ADAM10 and ADAM17 cleavage (1). Within the ECD, human Fractalkine shares 59% amino acid sequence identity with both mouse and rat Fractalkine. Fractalkine exists as both a membrane-bound adhesion molecule and as a soluble proinflammatory chemoattractant and anti-inflammatory neuroprotective agent (1-3). The expression of CX3CL1 is higher in spinal metastases from kidney cancer (4). The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes (5). Most of the functions of CX3CL1 are exerted through the CX#CL1/CX3CR1 axis which has the therapeutic prospect (5, 6). Our Avi-tag Biotinylated Recombinant Fractalkine features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
  1. Poniatowski, L. et al. (2017) Mol. Neurobiol. 54:2167.
  2. Desforges, N. et al. (2012) Int. J. Alzheimers Dis. 2012:345472.
  3. Nanki, T. et al. (2016) Mod. Rheumatol. 27:392.
  4. Liu, W. et al. (2016) Arch Immunol. Ther. Exp. (Warsz) 64:371.
  5. Zlotnik, A. Yoshie, O. (2012) Immunity 36:705.
  6. Quan, Z. et al. (2017) Current Gene Therapy 17:442.

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Recombinant Human
CX3CL1/Fractalkine Hi ...
AVI365
Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF
Species: Hu
Applications: Binding Activity, Bioactivity

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