Reactivity | HuSpecies Glossary |
Applications | Binding Activity |
Format | Carrier-Free |
Details of Functionality | Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 1-5 µg/mL. |
Source | Mouse myeloma cell line, NS0-derived human CLEC-1 protein Gln77-Asp280, with an N-terminal 10-His tag |
Accession # | |
N-terminal Sequence | His |
Protein/Peptide Type | Recombinant Proteins |
Gene | CLEC1A |
Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Endotoxin Note | <0.01 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 24.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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SDS-PAGE | 35-36 kDa, reducing conditions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Reconstitution Instructions | Reconstitute at 250 μg/mL in sterile PBS. |
CLEC-1 (C-type lectin-like receptor-1) is a type 2 transmembrane protein that belongs to the Dectin-1 family of C-type lectins (1, 2). It consists of a 52 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 207 aa extracellular domain (ECD) (3). The ECD contains a carbohydrate recognition domain (CRD) that includes cysteine residues with conserved spacing (4). In other C-type lectins, these cysteines form intrachain disulfide bonds that stabilize the CRD protein fold (4). CLEC-1 is a nonclassical C-type lectin in that its CRD lacks consensus amino acids which mediate calcium dependent carbohydrate binding in other C-type lectins (2, 3). The cytoplasmic domain contains one tyrosine, multiple serine and threonine, and a cluster of aspartic acid residues. The CLEC-1, CLEC-2, Dectin-1, and LOX-1 genes are located within the NK gene complex that also encodes several receptors involved in regulating NK cell activation (3, 5). CLEC-1 is most highly expressed in activated dendritic cells and at lower levels in endothelial cells and monocytes (3, 5). Within the CRD, human CLEC-1 shares 65% aa sequence identity with mouse and rat CLEC-1 and 26% - 36% with the other human Dectin-1 family lectins CLEC-2, CLEC9A, CLEC12B, Dectin-1, LOX-1, and MICL.
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