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Recombinant Human CD81 LEL Fc Chimera Protein, CF

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When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is used at 2 μg/mL, Recombinant Viral HCV E2 (Catalog # 9146-HC) binds with an ED50 of 0.2‑1.2 µg/mL.
When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is used at 2 μg/mL, Recombinant Viral HCV E2 (Catalog # 9146-HC) binds with an ED50 of 0.2‑1.2 µg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CD81 LEL Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human CD81 LEL Fc Chimera is used at 2 μg/mL, Recombinant Viral HCV E2 (Catalog # 9146-HC) binds with an ED50 of 0.2-1.2 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD81 protein
MD Human IgG1
(Pro100-Lys330)		 IEGR

Human CD81 LEL
(Phe113-Lys201)
Accession # P60033

N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36-41 kDa, reducing conditions
Publications
Read Publication using
9144-CD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD81 LEL Fc Chimera Protein, CF

  • CD81 antigen (target of antiproliferative 1)
  • CD81 antigen
  • CD81 molecule
  • CD81
  • CVID6
  • TAPA1
  • TAPA-1
  • Target of the antiproliferative 1
  • Tetraspanin-28
  • TSPAN28
  • Tspan-28
  • tspan-28,26 kDa cell surface protein TAPA-1
  • TSPAN28S5.7

Background

CD81, also known as TAPA-1 and Tetraspanin-28, is an approximately 25 kDa palmitoylated component of plasma membrane lipid rafts (1). It contains four transmembrane segments, two extracellular loops of 30 and 90 amino acids (aa), and three short cytoplasmic regions (2, 3). Within the large extracellular loop, human CD81 shares 90% and 84% aa sequence identity with mouse and rat CD81, respectively. CD81 associates with a wide range of membrane proteins including CD151, TfR2, LDL R, PCSK9, Glypican 3, IFITM1, IGSF8/CD316, FPRP, and complexes of CD19-CD21 (4-11). It is required for the development of CD4+CD8+ DP thymocytes (12) and hepatocyte infection by Plasmodium sporozoites (13). It also supports B cell receptor signaling (11), Hepcidin expression (5), monocyte and B cell tethering to the vascular endothelium (14), and the immunosuppressive function of Treg and MDSC (15). CD81 additionally functions as a receptor for the E2 glycoprotein of hepatitis C virus (16). The CD81-E2 interaction inhibits NK cell cytolytic activity, provides a co-stimulatory signal to T cells, and inhibits the maturation of plasmacytoid dendritic cells (17-19).
  1. Charrin, S. et al. (2014) J. Cell Sci. 127:3641.
  2. Oren, R. et al. (1990) Mol. Cell. Biol. 10:4007.
  3. Levy, S. et al. (1991) J. Biol. Chem. 266:14597.
  4. Zhu, Y.-Z. et al. (2012) Virology 429:112.
  5. Chen, J. and C.A. Enns (2015) J. Biol. Chem. 290:7841.
  6. Le, Q.-T. et al. (2015) J. Biol. Chem. 290:23385.
  7. Takahashi, S. et al. (1990) J. Immunol. 145:2207.
  8. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
  9. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:4853.
  10. Liu, B. et al. (2009) Am. J. Pathol. 175:717.
  11. Cherukuri, A. et al. (2004) J. Immunol. 279:31973.
  12. Boismenu, R. et al. (1996) Science 271:198.
  13. Silvie, O. et al. (2003) Nat. Med. 9:93.
  14. Feigelson, S.W. et al. (2003) J. Biol. Chem. 278:51203.
  15. Vences-Catalan, F. et al. (2015) Cancer Res. 75:4517.
  16. Pileri, P. et al. (1998) Science 282:938.
  17. Crotta, S. et al. (2002) J. Exp. Med. 195:35.
  18. Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
  19. Tu, Z. et al. (2013) Cell. Immunol. 284:98.

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Publications for CD81 (9144-CD)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Capture.


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