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Recombinant Human CCL1/I-309 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
272-I-010
Carrier Free
272-I-050/CF
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Recombinant Human CCL1/I-309 Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BW5147 mouse T lymphoma cells. The ED50 for this effect is 3-9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR8. The ED50 for this effect is 1.5-7.5 ng/mL.
Source
E. coli-derived human CCL1/I-309/TCA-3 protein
Lys24-Lys96
Accession #
N-terminal Sequence
Lys24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
272-I/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL1/I-309 Protein, CF

  • C-C motif chemokine 1
  • CCL1
  • chemokine (C-C motif) ligand 1
  • I-309
  • inflammatory cytokine I-309
  • P500
  • SCYA1
  • SCYA1Small-inducible cytokine A1
  • SCYA2
  • SISe
  • small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
  • T lymphocyte-secreted protein I-309
  • TCA3
  • TCA-3

Background

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.

CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The sequence of CCL1 contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15 - 16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

  1. Miller, M.D. et al. (1992) Proc. Natl. Acad. Sci. USA 89:2950.
  2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.

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Publications for CCL1/I-309/TCA-3 (272-I/CF)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, ELISA (Standard).


Filter By Application
Bioassay
(7)
Cell Culture
(1)
ELISA (Standard)
(2)
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Human
(7)
Mouse
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using 272-I/CF Applications Species
NS Haque, A Tuteja, N Haque CC chemokine CCL1 receptor CCR8 mediates conversion of mesenchymal stem cells to embryoid bodies expressing FOXP3+CCR8+ regulatory T cells PLoS ONE, 2019-07-17;14(7):e0218944. 2019-07-17 [PMID: 31314754] (Cell Culture, Human) Cell Culture Human
Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):. 2017-05-22 [PMID: 28533380] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Islam SA, Chang DS, Colvin RA Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011-01-09;12(2):167-77. 2011-01-09 [PMID: 21217759] (Bioassay, Mouse) Bioassay Mouse
Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM CC chemokine ligand 1 is released into the airways of atopic asthmatics. Eur. Respir. J., 2006-03-15;28(1):59-67. 2006-03-15 [PMID: 16540498] (Bioassay, ELISA (Standard), Human) Bioassay, ELISA (Standard) Human
Ruffini PA, Biragyn A, Coscia M, Harvey LK, Cha SC, Bogen B, Kwak LW Genetic fusions with viral chemokines target delivery of nonimmunogenic antigen to trigger antitumor immunity independent of chemotaxis. J. Leukoc. Biol., 2004-04-09;76(1):77-85. 2004-04-09 [PMID: 15075363] (Bioassay, Human, Mouse) Bioassay Human, Mouse
Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002-06-15;168(12):6244-52. 2002-06-15 [PMID: 12055238] (Bioassay, Human) Bioassay Human
Cashman J, Clark-Lewis I, Eaves A, Eaves C Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice. Blood, 2002-02-01;99(3):792-9. 2002-02-01 [PMID: 11806978] (Bioassay, Human) Bioassay Human
Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. J. Leukoc. Biol., 2001-10-01;70(4):617-23. 2001-10-01 [PMID: 11590199] (ELISA (Standard)) ELISA (Standard)

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Bioinformatics

Gene Symbol CCL1
Uniprot