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Recombinant Human Cathepsin V Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human Cathepsin V Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008). The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human Cathepsin V protein
Val18-Val334, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Val18
Structure / Form
Pro form
Protein/Peptide Type
Recombinant Enzymes
Gene
CTSV
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40 kDa, reducing conditions
Publications
Read Publications using
1080-CY in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Buffer: 25 mM Sodium Acetate, 0.1 M NaCl, 5 mM DTT, pH 5.5
  • Recombinant Human Cathepsin V (rhCathepsin V) (Catalog # 1080-CY)
  • Substrate: Z-Leu-Arg-AMC ((Catalog # ES008), 2 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhCathepsin V to 1 µg/mL in Assay Buffer.
  2. Dilute Substrate to 20 µM in Assay Buffer.
  3. Load into a black well plate 50 µL of 1 µg/mL rhCathepsin V and start the reaction by adding 50 µL of 20 µM Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

         Specific Activity (pmol/min/µg) =

    		 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
    amount of enzyme (µg)

         *Adjusted for Substrate Blank

         **Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC) (Sigma, Catalog # A-9891).

Per Well:
  • rhCathepsin V: 0.050 μg
  • Substrate: 10 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Cathepsin V Protein, CF

  • Cathepsin L2
  • Cathepsin U
  • Cathepsin V
  • CATL2
  • CTSL2
  • CTSUcathepsin L2, preproprotein
  • CTSVMGC125957
  • EC 3.4.22
  • EC 3.4.22.43

Background

Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.

  1. Turk, V. et al. (2001) EMBO J. 20:4629.
  2. Bromme, D. et al. (1999) Biochemistry 38:2377.
  3. Somoza, J.R. et al. (2000) Biochemistry 39:12543.

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Recombinant Human
Cathepsin V Protein, ...
1080-CY
Recombinant Human Cathepsin V Protein, CF
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Publications for Cathepsin V (1080-CY)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Enzyme Assay.


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Bioassay
(5)
Enzyme Assay
(2)
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Human
(4)
Mouse
(1)
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Showing Publications 1 - 7 of 7.
Publications using 1080-CY Applications Species
E Lindström, B Rizoska, I Henderson, Y Terelius, M Jerling, C Edenius, U Grabowska Nonclinical and clinical pharmacological characterization of the potent and selective cathepsin K inhibitor MIV-711 J Transl Med, 2018-05-09;16(1):125. 2018-05-09 [PMID: 29743078] (Bioassay) Bioassay
WJ Yi, MY Su, Y Shi, S Jiang, SZ Xu, TC Lei Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage Cell Cycle, 2018-04-25;0(0):1-40. 2018-04-25 [PMID: 29623762] (Bioassay, Human) Bioassay Human
JB Schaal, DQ Tran, A Subramania, R Patel, T Laragione, KD Roberts, K Trinh, P Tongaonkar, PA Tran, D Minond, GB Fields, P Beringer, AJ Ouellette, PS Gulko, ME Selsted Suppression and resolution of autoimmune arthritis by rhesus ?-defensin-1, an immunomodulatory macrocyclic peptide PLoS ONE, 2017-11-16;12(11):e0187868. 2017-11-16 [PMID: 29145473] (Bioassay) Bioassay
Jiang H, Cheng X, Shi G, Hu L, Inoue A, Yamamura Y, Wu H, Takeshita K, Li X, Huang Z, Song H, Asai M, Hao C, Unno K, Koike T, Oshida Y, Okumura K, Murohara T, Kuzuya M Cathepsin K-mediated Notch1 activation contributes to neovascularization in response to hypoxia. Nat Commun, 2014-06-04;5(0):3838. 2014-06-04 [PMID: 24894568] (Bioassay, Mouse) Bioassay Mouse
Zou F, Schafer N, Palesch D, Brucken R, Beck A, Sienczyk M, Kalbacher H, Sun Z, Boehm BO, Burster T Regulation of cathepsin g reduces the activation of proinsulin-reactive T cells from type 1 diabetes patients. PLoS ONE, 2011-08-05;6(8):e22815. 2011-08-05 [PMID: 21850236] (Enzyme Assay, Human) Enzyme Assay Human
Ma DH, Yao JY, Kuo MT, See LC, Lin KY, Chen SC, Chen JK, Chao AS, Wang SF, Lin KK Generation of endostatin by matrix metalloproteinase and cathepsin from human limbocorneal epithelial cells cultivated on amniotic membrane. Invest. Ophthalmol. Vis. Sci., 2007-02-01;48(2):644-51. 2007-02-01 [PMID: 17251461] (Bioassay, Human) Bioassay Human
Cheng T, Hitomi K, van Vlijmen-Willems IM, de Jongh GJ, Yamamoto K, Nishi K, Watts C, Reinheckel T, Schalkwijk J, Zeeuwen PL Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification. J. Biol. Chem., 2006-03-24;281(23):15893-9. 2006-03-24 [PMID: 16565075] (Enzyme Assay, Human) Enzyme Assay Human

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Bioinformatics

Gene Symbol CTSV
Uniprot